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What are the indole test principles?
The indole test is based on the ability of certain bacteria to produce the enzyme tryptophanase, which converts tryptophan into indole. This indole is then detected by adding a reagent such as Kovac's reagent, which reacts with indole to produce a red color. A positive test indicates the presence of tryptophanase enzyme in the bacteria.
What color is negative indole test?
A negative indole test typically shows no color change, indicating the absence of indole production. The medium remains yellow or unchanged, suggesting that the tested organism does not possess the enzyme tryptophanase, which is responsible for converting tryptophan to indole.
What is l-tryptophane?
L-Tryptophane is an amino acid that is made from plant or animal resources
What is the substrate for the Indole test?
The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to split indole from the amino acid tryptophan. This division is performed by a chain of a number of different intracellular enzymes, a system generally referred to as "tryptophanase."
What is the component in the SIM deep tubes that makes the medium suitable to detect the production of indole by bacteria?
The component in SIM deep tubes that makes the medium suitable to detect the production of indole by bacteria is tryptophan. Bacteria that possess the enzyme tryptophanase can break down tryptophan to produce indole, which can be detected by the addition of Kovac's reagent that reacts with indole to form a red color.
What enzyme changes glucose to frutose?
The enzyme responsible for converting glucose to fructose is glucose isomerase. It catalyzes the reversible isomerization of glucose to fructose. This enzyme is commonly used in the production of high-fructose corn syrup.
What is a protein that changes the rate of chemical reactions?
its called an enzyme
What is an indole alkaloid?
class of alkaloids containing a structural moiety of indole.
What is an enzyme called when it changes shape?
An enzyme is called a denatured enzyme once it changes its shape.
What does the S.I.M. test for?
Sulfur Indole Motility test
What test will differentiate between Proteus vulgaris and Citrobacter freundii?
Indole test,Proteus vulgaris is be indole positive and Citrobacter freundii will be indole negative
Changes in pH or temperature decrease enzyme activity because bonds break and the enzyme returns to its tertiary structure?
Changes in pH or temperature can disrupt the interactions that maintain the enzyme's specific shape, leading to denaturation and loss of enzyme activity. This is because enzymes are sensitive to changes in their environment, and alterations in pH or temperature can affect the enzyme's active site conformation, preventing it from binding to the substrate effectively.
