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the iris diaphragm controls the amount of light that passes through the stage and, consequently, through the specimen. Reducing the iris diaphragm aperture increases contrast for an image focused under high power by reducing the amount of light that both fills the objective lens and deracts around specimen edges. Opening the iris diaphragm under high magnification increases "flare", the appearance of light "washing out" an object. By decreasing the flow of light through the specimen, the iris diaphragm limits light defraction and saturation.
An iris diaphragm controls the amount of light admitted to a lens. Closing the iris diaphragm will decrease the brightness and increase the contrast of an image.
Cameras have an iris diaphragm.
The radial and circular muscles of the iris.
when observing a prepared bacterial smear, should the diaphragm be open or closed and why
The iris diaphragm adjusts the amount of light allowed in.
You have to adjust it to maximize contrast. If you have too much light, you won't have much contrast between the object and the background.
On a microscope, the iris diaphragm controls the amount of light.
The Iris
This iris diaphragm of a microscope contains the amount of light that can enter through to the specimen. If the condenser iris diaphragm is open, the image will be bright; if it is closed, it will be dim.
The diaphragm, often called the "iris" diaphragm for its identical form and function to the iris in your eye, regulates the physical amount of light allowed to pass through a camera lens. It may be called the "iris," the "diaphragm," the "iris diaphragm," the "stop," or the "aperture." All mean the same thing.Physically, the diaphragm or iris diaphragm is made up of overlapping thin opaque metal plates, usually six or eight, which can be adjusted to increase or decrease the diameter of the hole, called the stop, in the center of the lens. The diaphragm is often located at the optical center of the lens between sets of elements. On manual cameras there is usually a ring around the lens barrel marked in f/stops for adjustment of the diaphragm.The iris (diaphragm) in your eye, working properly, automatically opens or closes in response to light level. When you are exposed to strong light the iris closes down (or "stops" down) to a smaller diameter, decreasing the light reaching your retina (equivalent to the film or sensor in your camera). In dim light, the iris opens up to admit more light. The diaphragm in your camera works the same way, and on modern cameras it's often equally automatic. You may never even notice it's working.In a camera, the iris diaphragm and the shutter have to work together to control the total exposure. The iris diaphragm controls the amount of light, and the shutter controls the amount of light over time. Depending on the make and model of the camera, shutter and diaphragm may be manual, automatic or both. The diaphragm is the mechanism that varies the size of the aperture; the opening that allows light into the camera.
Robert Hooke