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What are disadvatages of chromatograph?

Updated: 8/21/2019
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10y ago

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chromatography has many varieties

-paper chromatography, sometime complexe mixtures cant be separated, TLC plates do not have long stationary phases

-gaz chromatography: the molecule should be volatile

-Chiral Chromatography can be expensive

- Ion Exchange or Ion Chromatography: Turbidity should be low below 10ppm

-Size Exclusion Chromatography: low resolution technique which gives few

peaks and requires large differences in molecular weight for resolution

-Gel chromatography: the target protein frequently becomes an abundant substrate for proteases that may also be present in the mixture. Another disadvantage is low sample handling.

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