proteins are expressed differently in prokaryotes and eukaryotes
the human protein coded for by the human gene
The cDNA (complementary DNA) encoding the eukaryotic protein must first be isolated from the cell prior to cloning. This involves reverse transcription of the messenger RNA (mRNA) and subsequent amplification to obtain the gene of interest for cloning into a bacterial expression vector.
The bacterium has been genetically modified to contain the human insulin gene. This gene allows the bacterium to produce insulin when it is transformed with the gene and given the appropriate conditions for protein synthesis.
Protein synthesis in eukaryotic cells primarily occurs in the cytoplasm. It involves the processes of transcription in the nucleus to produce mRNA, which is then transported out of the nucleus to the cytoplasm where translation takes place on ribosomes to produce proteins.
blaze
When plasmids are used to produce a desired protein, the gene encoding for the protein is inserted into the plasmid. The plasmid is then introduced into a host organism, such as bacteria, which then replicate the plasmid and express the protein. This allows for large-scale production of the desired protein.
1 is translation. 2 is restriction enzyme. 3 is prokaryotic transcription. 4 is DNA ligase. 5 is transformation. 6 is eukaryotic transcription. 7 is reverse transcription. So the order of the steps based off of the numbers are 6,7,2,4,5,3,1.
This type of protein is a histone.
Protein synthesis, also known as translation, is the process by which a protein is constructed in the cytoplasm of eukaryotic cells. This process involves the ribosome reading messenger RNA (mRNA) and assembling amino acids into a specific sequence to form a protein.
the web of protein fibers that help organize the parts of eukaryotic cell make up?
Ribosomes are the smallest between mitochondria, viruses, bacterium, and protein. Ribosomes are the building blocks of mitochondria, viruses, bacterium, and proteins.
Eukaryotic organisms transcribe intron regions when making mRNA molecules because they contain important regulatory sequences that help in the processing and splicing of the mRNA to produce a functional and mature mRNA molecule for protein synthesis.