stationary phase is the solid called the filter paper and mobile phase is the liquid or organic solvent present in the developing tank or beaker by ayesha zulfiqar
Paper is the (solid) stationary phase, 'sucking' up the liquid, mobile (= transporting) phase
mobile phase is the phase that consist of the analyte and stationary phase is the phase that is standstill
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
finding concentrations gas chromatogram of gasolineion chromatogram of Orange Juiceeach peak corresponds to a separate component in the mixturearea of each peak is proportional to concentrationchemical fingerprinting species identification "killer" bees can be distinguished from native bees by comparing gas chromatograms of cuticle extractstracing contraband sourcesdetecting drugs in urine
The mobile phase as indicated is the moving phase. Either the mobile or stationary phase is polar and the other is Non-polar. A common polar phase is Methanol, and non-polar is hexane
Bad things happen. The mobile phase evaporates and thus it's composition changes. The vapor phase above the chromatogram is not saturated or at equilibrium, and this affects the movement of the sample on the solid phase.
system volume is amount of mobile phase travel from reservior to tip of the colomn . dead volume is the amount of mobile phase present in colomn. delay volume is amount of mobile phase used in at the time of injection and the time of detection of chromatogram.
stationary phase is the solid called the filter paper and mobile phase is the liquid or organic solvent present in the developing tank or beaker by ayesha zulfiqar
Paper is the (solid) stationary phase, 'sucking' up the liquid, mobile (= transporting) phase
mobile phase is the phase that consist of the analyte and stationary phase is the phase that is standstill
The mobile phase as indicated is the moving phase. Either the mobile or stationary phase is polar and the other is Non-polar. A common polar phase is Methanol, and non-polar is hexane
For thin layer or paper chromatography, this can be a very important step, especially if one or more of the components in the mobile phase have very high vapor pressures (or low boiling points). Without the equilibration, as the mobile phase starts to move up the plate, the more volatile components will evaporate into the air space above the mobile phase faster than the less volatile ones. This is change the composition of the mobile phase (not much, but sometimes enough to change the Rf's of the unknown spots and standards). If the mobile phase consists of only water or perhaps high boiling solvents, the equilibration step is not as important. If the mobile phase contains Methylene chloride, Diethyl ether or similar solvents, then the equilibration step can be very important in terms of reproducibility.
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
finding concentrations gas chromatogram of gasolineion chromatogram of Orange Juiceeach peak corresponds to a separate component in the mixturearea of each peak is proportional to concentrationchemical fingerprinting species identification "killer" bees can be distinguished from native bees by comparing gas chromatograms of cuticle extractstracing contraband sourcesdetecting drugs in urine
a moving or mobile phase is a mixture you want to separate , dissolved in a solvent.
The mobile phase as indicated is the moving phase. Either the mobile or stationary phase is polar and the other is Non-polar. A common polar phase is Methanol, and non-polar is hexane
Normal Phase: It has a polar stationary phase and a non-polar mobile phase.Reverse Phase: It has a non-polar stationary phase and a moderately polar mobile phase