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Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?
# The pH will determine if the bacteria will have a particular charge. If the chromophore is a positive ion like the methylene blue in the equation shown in the reading, the stain is considered a basic stain; if it is a negative ion, it is an acidic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the negative charges of the bacterial cell.
Difference between basic and acidic stains?
Acidic oxides form acids with water: eg SO2 + H2O --> H2SO3 Basic oxides form basic salts (alkaline) with water: eg. CaO + H2O --> Ca(OH)2 Amfotheric oxides have both acidic and base properties: eg. Aluminium oxide is an amphoteric oxide, which means that it displays both acidic (1) and base (2) properties: 1. Al2O3 + H2O --> 2 Al(OH)2- + 2 H+ 2. Al2O3 + H2O --> 2 Al3+ + 3 OH-
Which of the following is an example of an acidic stain?
Basic dyes: Crystal violet, Methylene blue, Malachite green, Safranin. Acidic dyes: Nigrosin, Congo red
Difference between dye and stain?
A stain is temporary whereas dyes are permanent and can be removedonly after cell wall destruction.
What is the difference between laundry detergent and stain removers?
Laundry Detergent Gets Rid Of Smells And Odors And Stain Removers Get Stains Out.
What one of these leucocytes stain both acidic and bacic dyes?
Neutrophils stain both acidic and basic dyes
What are the differemt kind of stain?
Acidic,basic and neutral stains
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?
# The pH will determine if the bacteria will have a particular charge. If the chromophore is a positive ion like the methylene blue in the equation shown in the reading, the stain is considered a basic stain; if it is a negative ion, it is an acidic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the negative charges of the bacterial cell.
Difference between basic and acidic stains?
Acidic oxides form acids with water: eg SO2 + H2O --> H2SO3 Basic oxides form basic salts (alkaline) with water: eg. CaO + H2O --> Ca(OH)2 Amfotheric oxides have both acidic and base properties: eg. Aluminium oxide is an amphoteric oxide, which means that it displays both acidic (1) and base (2) properties: 1. Al2O3 + H2O --> 2 Al(OH)2- + 2 H+ 2. Al2O3 + H2O --> 2 Al3+ + 3 OH-
What is the difference between Wright stain and Giemsa stain?
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.
What is the basic for gram stain results between different bacteria?
its the difference in cell wall of both the type of bacteria
Is safranin an acidic dye?
Sure, any basic stain can be used for simple, direct staining.
What the principle of leishman stain?
It is basically use to stain leukocytes,maleria prasite and trypanosomas. leisman stain contain 1st methylene blue dye, a basic dye, which gives color to an acidic component.2nd eosin dye,an acidic dye ,which gives color to a basic component. These dye differentiat the different component of blood.
What is the principle of leishman stain?
It is basically use to stain leukocytes,maleria prasite and trypanosomas. leisman stain contain 1st methylene blue dye, a basic dye, which gives color to an acidic component.2nd eosin dye,an acidic dye ,which gives color to a basic component. These dye differentiat the different component of blood.
Why is size more accurate in an acidic or negative stain than in a simple or basic stain?
Since there is no heat fixing or strong cemicals are used the bacteria are less distorted
What is Sudan black b stain is use for in bone marrrow?
Sudan black B stainThis stain distinguishes between acute lymphoblastic leukemia (cells stain positive) and acute myeloblastic leukemia (cells stain negative).
Which of the following is an example of an acidic stain?
Basic dyes: Crystal violet, Methylene blue, Malachite green, Safranin. Acidic dyes: Nigrosin, Congo red
