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Neutrophils stain both acidic and basic dyes

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Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?

When methylene blue is prepared as a basic stain, it will have a positive charge and selectively bind to negatively charged components of bacterial cells, such as nucleic acids, enhancing the staining of bacteria. On the other hand, if prepared as an acidic stain, it will have a negative charge and repel bacterial cells, resulting in poor staining of bacteria.


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Leishman staining is used for staining blood in microscopy and its purpose is to both identify and differentiate trypanosomas, leucocytes and malaria parasites. Giesma staining is used to stain DNA region, specifically chromosomes in order to locate aberrations like rearrangement and translocations.


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Difference between basic and acidic stains?

Basic stains are positively charged and bind to negatively charged components in cells, resulting in a darker coloration of the cells. Acidic stains, on the other hand, are negatively charged and bind to positively charged components in cells. The choice between basic and acidic stains depends on the type of tissue being studied and the specific cellular components that need to be visualized.


Is Fe2O3 acidic or basic?

Fe2O3 is amphoteric, meaning it can act as both an acid and a base. In water, Fe2O3 can react to form both acidic and basic solutions depending on the conditions.


How do batteries and lemons relate?

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Which is a base vinegar or yeast?

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Which color is common to both the gram stain and the acid-fast stain?

The color common to both the gram stain and the acid-fast stain is red/pink. In the gram stain, Gram-negative bacteria appear red or pink after staining with safranin, while in the acid-fast stain, acid-fast bacteria such as Mycobacterium species retain the red/pink color of carbol fuchsin despite decolorization with acid-alcohol.