TaqMan Gene Expression Assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a FAM or VIC dye label on the 5' end, and minor groove binder (MGB) nonfluorescent quencher (NFQ) on the 3' end.
The recommended annealing temperature for performing thermal cycling in a PCR using TaqMan probes is typically around 60-65 degrees Celsius.
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A Numerical Expression is an expression only using numbers.
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The benefits are: for typing.