TaqMan Gene Expression Assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a FAM or VIC dye label on the 5' end, and minor groove binder (MGB) nonfluorescent quencher (NFQ) on the 3' end.
The recommended annealing temperature for performing thermal cycling in a PCR using TaqMan probes is typically around 60-65 degrees Celsius.
ATP (adenosine triphosphate) can be measured using various methods, with the most common being bioluminescence assays. These assays involve the enzyme luciferase, which catalyzes a reaction that produces light in the presence of ATP. The intensity of the emitted light is proportional to the amount of ATP present and can be quantified using a luminometer. Other methods include high-performance liquid chromatography (HPLC) and spectrophotometric assays, though bioluminescence is widely preferred for its sensitivity and simplicity.
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