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The cell walls are the difference. Gram stain will be positive, as the cell wall is thicker. Gram-positive bacteria retain the (crystal violet) dye. Then a decolorizer will be added and then the pink counterstain. This will give all gram-negative bacteria a red or pink coloring.

All the steps are done for all cultures being Gram stained and when they grow, the color will be seen as well as the shape.

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Lupe Hahn

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3y ago

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What is the difference between the gram-positive bacteria and gram-negative bacteria?

They have different cell walls.


What are the key differences between gram-positive and gram-negative bacteria in terms of their cell wall structure and response to Gram staining?

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What cell structure is significantly different between gram-positive and gram-negative bacteria?

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