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The scientific method 'per se' has nothing to do with distinguishing between "pure" substances and mixtures.

The scientific method is a procedure for approaching a problem objectively by:

1) observing and recording what is observed.

2) formulating an explanation for what is observed

3) thinking of a way to test the veracity of this explanation

4) performing this test (experiment)

5) writing up the results of the test for others to see.

If the test proves the explanation is incorrect then a new explanation is formulated and steps 3, 4 and 5 are repeated.

Thus while there are difference between pure substances and mixtures (see related question below) this is not directly related to the scientific method. However, the scientific method could be used to formulate a means of distinguishing between the two.

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Scientists make use of literally millions of substances and mixtures of substances, including elements, compounds, alloys, and various types of homogeneous or heterogeneous mixtures.

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Q: What are the different substances and mixtures in scientific method?
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