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One of the disadvantages of heating fixing is that it is very expensive. Secondly, it cannot be used in capsular staining.
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∙ 9y ago
Mustapha Abdul-Basit
Heating can also kill the the organism in-question
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What are the advantage and disadvantage of heat fixing?
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
What would happen if no heat fixing were done?
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
What is Passing a slide sample through a flame is called?
heat fixing
Does Heat fixing sterilize slide?
I guess so, and that's why you put out the flame after that.
What effect does heat fixing have on bacterial cell size?
it makes cell size shrink
What are the advantage and disadvantage of heat fixing?
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
Disadvantages of heat?
The disadvantages of heat are that if there is no solar energy, then there is no heat.
What would happen if no fixing were done?
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
What is fixing?
a heat fix is something you put on the slide
What will happen if too much heat is applied while heat fixing a slide?
When too much heat is applied during the heat fixing of a slide with a bacterial cell on it, the cell would explode. The membrane of the cell would rupture.
What are the advantages and disadvantages of positive ands negative staining for morphological study of bacteria?
Advantages: easy to carry out, natural shape and side Disadvatages: heat fixing can distorte cells, more attention needed
What would happen if no heat fixing were done?
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
Why is the slide not heat fixed before negative staining?
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
What is Passing a slide sample through a flame is called?
heat fixing
Does Heat fixing sterilize slide?
I guess so, and that's why you put out the flame after that.
What is the loss of carbon fixing activity of the enzyme RuBP carboxylase directly proportional to?
heat
What effect does heat fixing have on bacterial cell size?
it makes cell size shrink
