plant tissue culture or cell culture:-
it is the technique of in vitro, culture in which isolated plant cells, tissues, organs or even entire plant are subjected to grow in nutrient media in glass containers(tubes,conical flask,petri dishes) under aseptic conditions. Aseptic culture of plant may be of the following types as:-
(a) ORGAN CULTURE:- These are cultures of isolated plant organs including cultures derived from root tips,stem tips,leaf primordia or immature part of flowers and immature fruits.
(b) EMBRYO CULTURE:- These are culture of isolated immature or mature embryos.
(c) CALLUS OR TISSUE CULTURE:- These are culture of tissue arising from disorganized proliferation of cells from segment of plant organs.tissue or callus culture are , generally grown on solid medium as amass of cells.
(d) SUSPENSION CULTURE:- These are often called cell cultures, as they represent a lower level of organisation than tissue or callus culture. suspension culture are in vitro cultures of isolated cells and very small cell groups remaining dispersed as they grow in excited liquid media.
Plant tissue culture is a technique of producing clones of plant under controlled sterile condition. IN plant tissue culture, an explant is used to produce the whole plant in quick succession of time. Various steps include in the process are: * Prepare a suitable nutrient medium with carbon, nitrogen source along with the growth regulators. * Isolation of the explants (from the original plant) to be used. * Sterilization of the whole equipments like; vessels, media, explants, test tubes. * inoculation * incubation * hardening * Establishment in the field.
The basic technique in plant tissue culture is to exploit the totipotency of plant cells under aseptic conditions of optimum temperature and nourishment.
Asexual
ex plant are nothing but the part of plant that can be used for plant tissue culture processes like for the regeneration of hybrid plants and so on .
The functions of a plant tissue culture labaroatory is to clone plants by taking an explant off an already existing plant in order to get another plant with exatly the same genetic make up.
Asexual Reproduction
Using Tissue culturemany plant can be grown from one parent in disease-free conditions.
Callogenesis is the shoot development in callus, due to low auxin and hign cytokinin concentration in the culturing medium during tissue culture.
In plant tissue culture, cells of plants are cultured. In tissue culture, cells (of plants, animals, bacteria, etc.) are cultured. Plant tissue culture is just like a subheading under tissue culture
by tissue culture method
tissue culture of mango se.
ex plant are nothing but the part of plant that can be used for plant tissue culture processes like for the regeneration of hybrid plants and so on .
Lii Jang Liu has written: 'Tropical plant cell and tissue culture' -- subject(s): Plant biotechnology, Plant cell culture, Plant micropropagation, Plant tissue culture
Roberta H. Smith has written: 'Plant tissue culture' -- subject(s): Laboratory manuals, Plant tissue culture 'In Vitro Propagation of Kalanchoe (Avery's Plant Tissue Culture Series)'
Plant tissue culture usually takes some time to grow. Depending on the culture taken, it can take a couple weeks.
using tissue culture many plant can be grown from one parent in disease free condition
plant tissue culture
S. S. Bhojwani has written: 'The embryology of angiosperms' 'Plant tissue culture' -- subject(s): Plant tissue culture, Bibliography
The functions of a plant tissue culture labaroatory is to clone plants by taking an explant off an already existing plant in order to get another plant with exatly the same genetic make up.
Cloning of crop plants in tissue culture involves tiny pieces from the parent plant. Sterile agar jelly with plant hormones are needed which makes tissue culture an expensive way of cloning crop plants.