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Answered 2011-03-04 01:19:37

The dye can allow us to view the DNA after separation from electrophoresis. Without the dye, it is hard for us to see how far fragments of DNA traveled from their original location.

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What are the functions of the loading dye in electrophoresis?

First, loading dye is meant to help weigh down the DNA solution, so that it can sink into the bottom of the wells and not float in the buffer solution.Second, two different types of loading dye are used in electrophoresis. One of them moves more quickly than most of your DNA fragments, and the other moves more slowly, helping you determine the relative position of your DNA, as it should be in between the two bars or dye. This also tells you when to stop electrophoresis so that your DNA does not fall out of your agarose gel.Note that loading dye does not bind to the DNA itself and does not allow you to see the bars of DNA usually seen in a complete DNA fingerprint.

What tell the computer how to perform the functions of loading storing and executing an application program and how to transfer data.?

_ tell the computer how to perform the functions of loading, storing, and executing an application program and how to transfer data.

What is the purpose of a stain in electrophoresis?

I think the Question should be " What is the use of a dye in electrophoresis?" to be more specific, The normal dyes that are incorporated into the sample loading buffer in case of agarose gel is bromophenol blue and xylene cyanol.These are used to track the movement of the sample, so that we can stop the electrophoresis when the dye front is nearly 80%(can vary depending on the molecular weight of the sample) away from the well. In case of polyacrylamide gel electrophoresis the dye has many functions: gives density to the sample so that it sits properly in the well. SDS binds to the amino acid and makes it anionic, so that the movement of the sample is based on molecular weight, if not the charge of the protein will also influence the movement. it also helps us to track the movement of the sample. DTT or mercaptoethanol helps in the breaking of cysteine bonds.. That's it.. But the question is too broad and can be specific!!!!!!!!!1

What are the advantages and disadvantages of PHP functions?

There are too many (hundreds) functions for there to be an answer to this. Advantages are that they can be used server side to print output that can be translated from databases etc. Disadvantages it that it can delay the loading time of all you pages.

What is dynamic loading?

loading which cause movement of the object or structure is called dynamic loading

What Is Carbo-Loading?

Carbo-Loading Is When You Eat A Lot Of Food Containing Carbohyrdates

What chemical is used for loading?

Allum is used for loading

What is the old name of Cyprus?

Page 72 is not part of this book preview Loading... Loading... Loading... Loading... KITTAM. according to the old testament Ezk 27:6

What is program loading?

a program loading is when the program is generating its information

Phosphate loading sport banned?

Phosphate loading is legal.

When was The Loading Zone created?

The Loading Zone was created in 1966.

Function of agarose in agarose gel electrophoresis?

Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to make particles move. For example, DNA is negative, so it'll travel towards to positive electrode of the gel box. Agarose has small pores through which a DNA can travel. Bigger fragments of DNA travel shorter distances, because it takes longer for them to navigate through the pores of the agarose gel. Identically sized pieces of DNA will travel the same distance, which is why you get bands (DNA with loading dye) after you run a a gel.

Loading and running a diagnostic disk?

Loading and running a diagnostic disk?

Floor loading calculation of a battery?

Floor loading calculation of a battery

Can you use a loading place for shopping?

Loading zone is for deliveries only

What is difference between bar and beam?

bar can take axial loading only and beam can take both axial loading and transverse loading

How does the specific magnetic loading effect the design of electrical machine?

The magnetic loading and electrical loading is most important parameter in the electrical machine. the electrical machine's torque is define as T=winding factor*electrical loading*magnetic loading*rotor volume You can see that the magnetic loading can decide electrical machines volume so this parameter is important.

What is the difference between between direct loading and phantom loading?

In phantom loading,less power is consumed as the current and voltage coils are connected seperately;but in direct loading a load is connected which draw a considerable power.

When was The Loading Program created?

The Loading Program was created on 2003-09-09.

Are front loading washers more efficient than top loading?

Efficiency has next to nothing to do with front or top loading washers, but has everything to do with how good the technology is.

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