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The light is reflected upwards, and passes through the specimen. It passes through easier (clearer) if the specimen is not too thick.
because the specimen is always thin
because the thinner it is the clearer it is too see inside
microscope
A transmission electron microscope (TEM) is a microscope in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through it.
Light microscope works because light goes *through* your specimen. So if the specimen is too thick, then light won't shine through, and you won't see anything.
The stage in a microscope holds the specimen, usually on a slide, and has an opening in it to permit light to come up through the stage and through the specimen and further on up to the optics.
The specimen prepared for a monocular microscope must be very thin so light can pass through it easily. The light then goes through a series of lenses that magnifies the specimen to appear bigger
A sample is collected through the urine. The specimen collection is called a '24 hour' collection, according to this website. I hypothesize that this period of time is a gestation period to allow the potassium in the urine to either grow or 'collect'; however, one must ask how the potassium is stored for this period. In refrigeration, or room temperature?
So you do not forget and to describe it accurately to others who have not looked at the specimen through the microscope
Longitudinal section.
It regulates the amount of light that passes through a specimen.