What are the limitations of a UV Visible Spectrometer?
What are the limitations of a UV Visible Spectrometer
UV-Vis refers to ultraviolet-visible spectroscopy, where UV-Vis measures absorption or reflectance in the ultraviolet-visible spectral range. Using light in the visible and adjacent ranges, such as UV and IR, excites molecules to undergo electronic transitions. UV-Vis measures ground state to excited state transitions.
It is a spectra that shows how transparent a material can be. in scientific terms, it is incidence of electromagnetic radiation (from the UV, Visible, Infrared wavelength regions) from a source to a material, and there is a detector which records the amount of radiation remaining after the transmission (these radiation that is gone is either reflected of the surface or absorbed by the material). The equipment that measures this is called UV-Vis-NIR spectrometer.
The only difference, between ultra violate light and visible light, is their frequencies, UV has higher frequency than visible and since wavelength x frequency = the speed of light (which is constant at 3x108 ms-1), therefore wavelength is higher in UV. UV light is also not visible, unlike visible light! so, in summery the difference is frequency and wave length.
If you compared the frequencies of UV light an visible light you and rsquod find that the UV light had a smaller frequency.?
The Beer-Lambert law Absorbance = (extinction coefficent)(pathlength of light)(concentration) allows you to measure the absorbance of sample in a UV spec, and change the rate from absorbance units / time to change in concentration / time. the pathlength of light being the width of the cuvette and the extinctin coefficent being specific to the product molecule.