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Q: What are the most important characteristics of compounds that effect their gas chromatography separation?
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What can chromatography be used for?

The uses of chromatography are important in checking the purity of oils extracted from plants, which are often used in the medicine industry. It shows all the different colours of chemicals in the mixture can be used to compare the elements of two mixtures.


The relative vapor pressures of the mixture components are important in GC Gas Chromatography but not in LC Liquid Chromatography Why is that?

In Gas Chromatography, before you can correctly separate and identify the components, you need to vapourise the mixture first. How fast a substance vaporises is dependent on their vapour pressure. In Liquid Chromatography, there is no need to vapourise the mixture and hence vapour pressure is not important here.


Uses for paper chromatography?

They are used in many scientific studies to identify unknown organic and inorganic compounds. They are also used in crime scene investigation, DNA and RNA sequencing, among others. Essentially, any solution can be separated through some form of chromatography.


Why do chromatography?

Chromatography is the methods used to separate complex mixtures. The components to be separated are distributed between two phases, a stationary and mobile phase. There are many types of chromatography: Liquid Chromatography, Gas Chromatography, Thin-layer Chromatography, and Paper Chromatography. As the separation occurs, a mixture is separated into its components. As a result, the molecules of the components have different masses, and so they travel along a medium at different rates. The process of chromatography can be used on a T-shirt. The mobile phase would be the alcohol and the stationary phase is the permanent markers. The solvent is the alcohol and the solutes are the marks made by the permanent markers. When the solvent, in this case, the alcohol, is distributed onto the shirt, some of the marks dissolve in the solvent. After a period of time, the marks on the medium, the T-shirt, end up spread out between the original spot and the point the solvent reaches. In designing the shirt, the alcohol spread the marker making concentric rings of different colors. This effect of concentric rings occurs since the different components of the permanents markers travel at different rates. In any chemical or bio-processing industry, the need to separate and purify a product from a complex mixture is a necessary and important step in the production line. For example, pharmaceutical industry uses chromatography to isolate penicillin and other antibiotics. Proteins can even be separated into amino acids through the process of chromatography. Chromatography is also used in crime scene investigation for DNA and RNA sequencing as well as in many scientific studies to identify unknown organic and inorganic compounds. This separation of mixtures is useful to us in various ways.


Why is it important to avoid air bubbles in the column during chromatography?

It is important to avoid air bubles in the column during chromatography because you get a clearer picture and will see what ishappening alot clearer without problems.


Why it is said that number of theoretical plates should be high for a good efficiency?

In liquid chromatography the 'theoretical plates' number is a measure of the resolution between the peaks of different eluting substances. The higher the plate value the greater the separation. This is particular important as the load reaches the maximum the column is designed for.


How is volatility important for the separation of components of a mixtures?

its not.


Is one of the most important compounds necessary for life?

Water is one of the most important compounds neceesary for life.


How is chromatography used in DNA mapping to identify a criminal in a murder case?

Chromatography is an important tool in DNA mapping. It can be used to match any DNA to previous DNA that is already on record from other crimes.


What are most important characteristics of a survivor?

i think the most important characteristics is experience and endurance


Is separation techniques important in your daily life?

yes


Use of gas chromatography in pharmaceutical industry?

Chromatography is a laboratory process that occurs in several steps and is used to separate mixtures of various chemicals into their individual components. The governing principle of chromatography is that different chemicals in a mixture have different degrees of dissolving in a liquid or sticking to a solid surface. In other words, chromatography can identify a chemical and separate it from a dense mixture of other chemicals and show it on a surface. Various chemicals in a mixture have different sticking ability on a surface. By varying this process in many ways, the chromatography technique can be used to separate any amount of quantities ranging from micrograms (in laboratories) to tons (in chemical plants). There are various chromatography procedures that have become popular since the invention of chromatography by Russian botanist, Mikhail Semyonovich Tsvet, in 1901. In the chromatography processes, a stream of liquid, that is called mobile phase, is made to flow through a tube known as column, and it is packed with porous solid material, called the stationary phase. The sample of the mixture that is to be analyzed is sent through the mobile phase and as the mixture proceeds in the tube, the compounds are separated. Chromatography is preferred over many other techniques as it doesn't cause any molecular changes in the composition of the chemicals involved. Uses of Chromatography Chromatography has evolved to be one of the most widely used chemical techniques to separate particles and contaminates in chemical plants. For example, in the chemical industries, pesticides and insecticides like DDT in the groundwater and PCBs (Polychlorinated biphenyls) are removed by the process of chromatography. As a major testing tool, chromatography is used by government agencies to separate toxic materials from the drinking water and also to monitor air quality. Chromatography is used by pharmaceutical companies to prepare large amounts of pure materials that are further required in making medicines. Also, it is used to check the presence of any contamination in the manufactured compounds. In the field of organic chemistry and pharmacy, chiral compounds are very close to each other in terms of atomic or molecular weight, element composition, and the physical properties. However, they exist in two different forms, called the enantiomers and optical isomers. Both these compounds though may appear to be same, have very different chemical properties. So, in pharmacy, chromatography becomes crucial to analyze the exact chiral compound so that correct medicines can be manufactured. For instance, a compound called thalidomide has two optical isomers and one of the isomers can cause birth defect if a pregnant women consumes it in early stages of pregnancy. So, it is important to carefully separate the isomers. Chromatography is used as a technique to separate the additives, vitamins, preservatives, proteins and amino acids. Some other uses are in the detection of drugs or medications in the urine and the separation of traces of chemicals in the case of fire in houses or buildings. It is also very popular in forensic science for investigative purposes. Chromatography technology has gained immense industrial popularity in the past few decades as it can separate chemicals that just differ even in their atomic orientations in space.