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What is the difference between the stationary phase and the mobile phase in chromatography?
Wiki User
∙ 11y ago
stationary phase stays at the bottom of the paper chromatography while mobile phase is moving on the stationary phase and move on stationary phase till it gets its right place on the top of the paper or somwhere else.
Wiki User
∙ 11y ago
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Principle of partition chromatography?
Chromatography in which separation is based mainly on differences be- tween the solubility of the sample components in the stationary phase (gas chromatography), or on differences between the solubilities of the compo- nents in the mobile and stationary phases (liquid chromatography)
What is mobile and stationary phase in Chromatography?
Moblie phase is that phase in chromatography that moves the analyte components along with it. Stationary phase remains static during chromatography.
What is the difference between partition chromatography and adsorption chromatography?
The partition chromatography involves separation between liquids while adsoption chromatography involves solid and liquid separations. Answer: Partition Chromatography is a liquid liquid extraction which involves two solvents while adsorption chromatography is a liquid solid extraction which involves a solid stationary phase & a liquid mobile phase.
What difference between dad and uv detector in hplc?
Liquid chromatography (LC) encompasses all chromatographic techniques using liquid mobile phase, including planar chromatography (paper chromatography and thin-layer chromatography) and column chromatography (classical column chromatography, and high-performance liquid chromatography on packed and capillary columns). The term liquid chromatography is nowadays often used as a sinonim for high performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC).
Why chromatography is a physical process?
Chromatography is physical techniques for separation. The method involves the dissolution of the materials in a mobile phase, then forced through a stationary phase in which the components of the sample separate.
Principle of partition chromatography?
Chromatography in which separation is based mainly on differences be- tween the solubility of the sample components in the stationary phase (gas chromatography), or on differences between the solubilities of the compo- nents in the mobile and stationary phases (liquid chromatography)
What is mobile and stationary phase in Chromatography?
Moblie phase is that phase in chromatography that moves the analyte components along with it. Stationary phase remains static during chromatography.
What is the difference between partition chromatography and adsorption chromatography?
The partition chromatography involves separation between liquids while adsoption chromatography involves solid and liquid separations. Answer: Partition Chromatography is a liquid liquid extraction which involves two solvents while adsorption chromatography is a liquid solid extraction which involves a solid stationary phase & a liquid mobile phase.
What difference between dad and uv detector in hplc?
Liquid chromatography (LC) encompasses all chromatographic techniques using liquid mobile phase, including planar chromatography (paper chromatography and thin-layer chromatography) and column chromatography (classical column chromatography, and high-performance liquid chromatography on packed and capillary columns). The term liquid chromatography is nowadays often used as a sinonim for high performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC).
What is a definition of chromatography?
It is a technique based on the general principle of distributing components of a mixture between stationary and mobile phase.
What are the principles and methods of high performance liquid chromatography and gel chromatography?
Liquid chromatography separation of multicomponant system according to diffrent interaction of dissolved componant with stationary phase. the stationary phase and mobile phase is very wide range
What is the difference between a mobile source and a stationary source?
Well, a mobile source is a source that is capable of moving. And a stationary source is a source that stays still or doesn't move (or stationary in other words).
Why chromatography is a physical process?
Chromatography is physical techniques for separation. The method involves the dissolution of the materials in a mobile phase, then forced through a stationary phase in which the components of the sample separate.
How does chromatography separate molecules?
The principle is based on equilibrium of the molecules between the mobile phase and the stationary phase. This will vary depending on the polarity of the substance, and the two phases. If the substance in more soluble in the mobile phase, it will travel further, and vice versa.
What is the significance of TLC over paper chromatography?
Paper chromatography and TLC both use a solid stationary phase and liquid mobile phase. The only real difference is in the physical and chemical composition of the stationary phase. Paper chromatography uses a fibrous protein/polymer mesh (i.e. paper) as its stationary phase, TLC uses a compacted powder (usually silica or alumina) on a sealed backing.
What is Chromatography and what is it for?
Chromatography is an analytical method to separate the compounds from the two immiscible phases,one being a mobile phase and another being a stationary phase. It is used in petroleum industry,pharmaceutical industry.
What are the advantages and limitations of column chromatography over thin layer chromatography?
TLC. The mobile phase is a liquid, the stationary phase is a solid. Useful for seperating and comparing mobility of solids and some liquids dissolved in the mobile phase by their affinities to the solid phase relative to the mobile phase. GLC. The mobile phase ia s gas, the stationary phase is a liquid on a solid support. same concept as TLC. useful for seperating gases by their affinities to the stationary phase...the mobility can then be compared to known compounds for possible identification.
