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Replication, Transcription, and Translation
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∙ 15y ago
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What are the 3 rules that DNA replication must follow?
DNA cannot replicate de novo (i.e. there must be a free 3' OH group to attach nucleotides to) Replication must go from 5' to 3' The two strands of DNA are antiparallel Complementary base pairing
The direction of DNA replication on the leading strand occurs in the?
The enzyme DNA polymerase synthesises strands in the 5 prime to 3 prime direction, and as DNA is antiparallel the replication of the leading strand occurs from the 3 prime end of the template to the 5 prime end of the template.
What is the 2nd step of DNA replication?
1. DNA unzips 2. Nitrogen bonds floating outside of the nucleus attach with the unzipped DNA. 3. Two DNA strands with the exact same bases are formed.
What is the function of DNA ligase in DNA replication?
it ties the chunks of dna together
Flow chart of 8 steps of DNA replication?
The eight steps of DNA replication are: 1. DNA strands separate, 2. formation of replication fork, 3. RNA primase binds, 4. bases pair up, 5. elongation, 6. RNA primers removed, 7.termination, 8. repair. this can occur in any cell.
What percentage of DNA will be radioactive after 3 DNA replication cycles?
3%
During DNA replication DNA polymerase lands at origination sites and forms?
DNA polymerase is instrumental in DNA elongation as it catalyzes the addition of deoxyribonucleoside triphosphates to the 3 prime end hydroxyl group of the DNA chain. DNA polymerase binds on the origin of replication, and forms a pre-replication complex with other proteins. The replication complex unwinds DNA during replication
What happens During replication DNA .?
During DNA replication the following occurs: 1) An enzyme called helicase separates the DNA strands (the space where they separate is called the replication fork). 2) DNA polymerase adds complementary nucleotides to the separated strand of DNA. 3) The DNA polymerase enzyme finishes adding nucleotides and there are two identical DNA molecules.
Process by which DNA copies itself?
Replication! 1) DNA splits 2) DNA copies 3) left with 2 copies of DNA! :)
What are the 3 rules that DNA replication must follow?
DNA cannot replicate de novo (i.e. there must be a free 3' OH group to attach nucleotides to) Replication must go from 5' to 3' The two strands of DNA are antiparallel Complementary base pairing
Difference between DNA replication and transcription?
1. Replication is the duplication of two-strands of DNA. Transcription is the formation of single, identical RNA from the two-stranded DNA. 2. There are different proteins involved in replication and transcription. 3. In replication, the end result is two daughter cells, while in transcription, the end result is a protein molecule. 4. In transcription, DNA serves as the template for RNA synthesis.
Why does DNA replication have to be in an anti-parallel direction?
DNA polymerase III can only work in the 5' to 3' direction.
What is the job of the DNA polymerases?
DNA polymerase adds bases to the 3' end during replication. It matches the c with G and A with U during replication. Never add to the 5' end!
What end of the DNA molecule allows the best access for DNA polymerase during replication?
3'OH end 3'OH end
The direction of DNA replication on the leading strand occurs in the?
The enzyme DNA polymerase synthesises strands in the 5 prime to 3 prime direction, and as DNA is antiparallel the replication of the leading strand occurs from the 3 prime end of the template to the 5 prime end of the template.
What is the role of DNA ligase in the enlogation of the lagging strand during DNA replication?
it is a linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of one DNA fragment to the 5' end of another DNA fragment.
What are the 3 models of DNA replication?
Conservative replication would leave intact the original DNA molecule and generate a completely new molecule.Dispersive replication would produce two DNA molecules with sections of both old and new DNA interspersed along each strand.Semiconservative replication would produce molecules with both old and new DNA, but each molecule would be composed of one old strand and one new one.
