Gram stains are used to analyze bacteria and determine their characteristics. The most popular use of a gram stain is to determine the thickness of a cell wall in bacteria.
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
This is a Gram Stain. If the technique was proper, the red rods are Gram-negative and the purple cocci are Gram-positive. This staining technique is used to help identify various bacteria. The Gram-positive bacteria that are purple hold the stain due to it's layered cell membrane. It contains a peptidoglycan layer that acts as a lattice trapping the crystal violet-Iodine dye complex.
armagheddon
Describe the Gram stain technique and the effect on Gram-positive and Gram-negative bacteria after each step. Be very specific about what is happening at each step and why it happens. (hint: be sure to fully explain your answer and not just list the steps)
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
A gram stain is a cow
The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.
Gram stain
Gram Negative
Protists are often stained using a silver stain, not a Gram stain.
A basic dye used in gram staining is crystal violet.
The Gram stain is used for bacteria and not for viruses.