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Some of the things that disrupt the three dimensional structure (or confirmation) of a protein are:

  1. Extremes of pH
  2. Very high temperatures
  3. Repeated freezing and thawing
  4. Placing the protein in an organic solvent (only if the protein has a hydrophobic interior)
  5. Placing the protein in a high salt solution
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15y ago
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15y ago

Heating unfolds proteins because most of the forces that maintain folding are weak attractions between pairs of side groups, and between side groups and water.

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15y ago

Several things, but most notably proteins are denatured by heat and chemicals.

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11y ago

Heat and chemicals

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Q: What are two things that will destroy a protein?
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