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Helium will vibrate your vocal chords, like "normal" air does, but since helium is lighter than the air we normally breathe and has different properties it causes the vocal chords to resonate at a much higher pitch. That's why people sound as if their voice is a sped-up recording when they talk after breathing it.

Though it's amusing on occasion, breathing helium in regularly probably is not a good idea. It might not permanently damage the vocal chords, but anything breathed into the lungs will likely eventually make it into the bloodstream and then the veins. You don't want helium to replace the oxygen that normally travels to your brain. In fact, if you were in a sealed room filled with more helium than normal air you would probably suffer brain damage or death from oxygen deprivation.

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Q: What can heilum do to your vocal cord?
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