for the starch iodine test is performed in which the starch reacts with iodine to produce dark blue colour which confirms the presence of starch. for glucose benedict's and fehling's test is performed. benedict's test: 1 ml sample is mixed with 1 ml of benedicts solution and the heated upto boiling if the colur changes to brick red then it confirms the presence of glucose fehling's test: similarly 1 ml fehling's solution I & fehling's solution II each in taken together and to it 1 ml of the sample is takenon then heated uptill boiling. if the colour changes to brick red the it confirms the presence of glucose.
Biuret solution turns a shade of purple if proteins are present. Specifically, it turns a shade of purple if peptide bonds are present. A peptide bond is the bond formed between amino acids.
Funny cause I actually had this question on my IB biology test today. The least important function of proteins is hormones at least out of the choices on my test.
1. Add 5 cm3 dilute sodium hydroxide (caustic!) to a 1% solution of albumen (the white part of an egg)2. Then add 1% copper sulphate solution3. A purple colour appears and this indicates that protein is present.
starch
prosrdure of benidect solution test
Sodium carbonate is added to increase the pH of the solution.
Most chemical supply companies sell it. You can do an internet search for "retail sale of Benedicts solution".
Benedicts solution
It is normally benedicts solution. Add about 1/4 of the total sugar solution to a test tube and heat it in a hot water bath at around 50 degrees C for 5 min. For example, heat 40mL of sugar water (C12H22O11 aq ) with 10mL of Benedicts soluton (copper and sodium salts). If it is a positive test it will turn yellow.
Simple sugars: Benedict's solution test for reducing sugars. Starches: Iodine test, which turns blue-black in the presence of starch. Lipids: Sudan IV test, where lipids turn a red color. Proteins: Biuret test, leading to a color change from blue to purple in the presence of proteins.
A reducing sugar such as glucose can be oxidized by both Benedicts solution and Tollens reagent to form a colored precipitate. This reaction is used to test for the presence of reducing sugars in a solution.
The test for proteins is called the Biuret test. This test is based on the principle that proteins react with copper sulfate in an alkaline solution to produce a violet color.
To test for carbohydrates in foods, you can use Benedict's solution for reducing sugars, iodine solution for starch, and glucose test strips for glucose content. For lipids, a grease spot test or Sudan III stain can be used to detect fats. Proteins can be tested for using Biuret reagent, which turns purple in the presence of protein. Lastly, you can test for nucleic acids with diphenylamine reagent, which turns blue in the presence of DNA or RNA.
The biuret test can be used to show the hydrolysis of proteins. In this test, a blue to purple color change indicates the presence of peptide bonds being hydrolyzed. This color change occurs due to the formation of a coordination complex between copper ions and the peptide bonds.
penis
Benedicts solution comprises of solution A(copper sulphate) and solution B(sodium pottasium citrate).chemically it is used as a test for aldehydes or generally carbonyl group.It is used to measure the sugar(allose,glucose,mannose,gullose,indose,galactose,tallose)content in food.-santa