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Q: What causes to get less bacteria on bacterial plate?
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How sound effect bacterial growth?

Sound significantly slows bacterial growth. In an experiment conducted in my college biology class, 6 dishes full of nutrient agar (bacteria food) were swabbed with bacteria from a students mouth and then closed and left to grow with music playing next to it. The bacteria that had music grew much, much less bacteria in the allotted time.


What is the purpose of bacterial staining?

1. It is rapid method of staining 2. useful in case of sterile samples like CSF pleural fluid etc where no. of pus cells and bacteria will be less


Why are viral infections more difficult to treat than bacterial infections?

That is a difficult one. It depends on the virus and the bacteria. Most viruses cause self-limiting diseases, that are treated with supportive measurements. Other viruses, such as Influenza, Cytomegalovirus and Herpes, and HIV, can be treated with specific drugs. There are different drugs that treat different types of bacteria, with more or less success. But remember that viruses and bacteria are live organisms, and they can and do develop resistance to the drugs that kill them. Even more, bacteria can even pass on the learned resistance to other bacteria.


When would a microbiologist want to use the streak-to-grow method.?

Plate streaking is often done to isolate a colony of bacteria. For instance, if a broth was grown with 2 or 3 different types of bacteria, it could be streaked out in a "3-phase streak." In a 3-phase streak the initial streak takes up a very small area (we draw a T on the back of the plate, this streak goes in the section above the T). The loop is flamed to kill off any bacteria still on it, then a couple streaks are made out of the original. This grabs some of the bacteria from that concentrated streak and spreads them out. This is repeated once more until the final streaks are less and less concentrated bacteria. When we incubate the plate we'll find lots of growth where the original streak was and less and less as we follow the path of the 3-phases. What you're looking for now is a single colony, off by itself. This can then be scraped off and plated on a separate plate and considered a "pure colony." Another usual time to use streak-to-grow bacteria is when you want to know the quantity or concentration of bacteria. You take maybe 0.1mL of solution and plate it, then count the number of colonies that form. Say you have 56 colonies, now you can say: 56 cfu (colony forming units) -------- 0.1 mL or 560 cfu/mL This is usual when testing to see whether a sterilization technique worked, or if a product is within the regulated levels of bacteria to be released the public, etc etc.


Do both the spread-plate and pour-plate method in a experiment produce similar bacterial counts or are they vastly different?

Both Spread-plate and pour plate method don't give the same results. Because in the case of spread plate method the inoculmn used for inoculation can't be spread in a exact volume. A little inoculmn remains stick with the spreader after spreading. On the other hand, in pour plate method it doesn't happen. So mostly, through comparing the counts by both methods, less counts are obtained in spread plate method. I am Working as a Sr. Microbiologist in a Biotech company

Related questions

Preservatives such as citric acid are added to foods to interfere with bacterial growth This creates an acidic pH in the food Why does this affect the bacteria that are present?

An acidic environment causes bacterial enzymes to fail or work less efficiently.


What is the main advantage of pour-plate method over other methods of bacterial colony isolation?

Previous answer: "because of infection" This person is obviously trying to be funny, the right word should be "Contamination", as for spread plate, the bacteria is more exposed to air as it is spread over the agar plate. Therefore, the result might not be accurate, as it might be contaminated. As for the pour plate method, the bacteria is in the agar itself, it is not exposed to air, thus, less risk of getting contaminated.


What is the difference between bacterial cells and animales cells?

bacteria is uni- cellular and less complex and also bacteria cells dont have a nucleus. the mitochondris stores the DNA and RNA.


Do dimes have more bacteria than pennies?

Actually copper, nickel and silver help to inhibit bacterial growth, therefore coins are far less likely to carry numerous bacterial colonies.


How sound effect bacterial growth?

Sound significantly slows bacterial growth. In an experiment conducted in my college biology class, 6 dishes full of nutrient agar (bacteria food) were swabbed with bacteria from a students mouth and then closed and left to grow with music playing next to it. The bacteria that had music grew much, much less bacteria in the allotted time.


What is bindoclav use for?

Bacterial infections in various parts of the body. It's an antibiotic combined with a drug that makes bacteria less resistant to the antibiotic.


What pathogen can be killed by antibodies?

Bacteria are made less harmful with antibodies by either killing the bacterium outright, or by weakening the bacterial production and letting the body's natural systems destroy the bacteria.


What causes a fishy order with no discharge?

A fishy odour is a sign of an overgrowth in bacteria.This can occur as a result of a temporary imbalance in pH, for example after penis-to-vaginal intercourse without a condom as the semen is more alkaline it makes the vaginal pH less acidic so more favourable to harmful bacteria that can cause this fishy odour. If the odour continues for a few days it may be a bacterial infection like Bacterial Vaginosis, where the harmful vaginal bacteria takes over from the healthy vaginal bacteria - this requires a trip to your doctor.


Can bacteria causes disease?

Less then 1 percent cause disease. Disease causing bacteria produce toxins- powerful chemicals that damage cells and make you ill.


He bacteria in a colony are unable to perform transduction. How would this hurt the bacterial colony's chance for survival?

the bacteria would be more genetically similar.


Advantages of pour plate method over other methods of bacterial colony?

The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.


What is the purpose of bacterial staining?

1. It is rapid method of staining 2. useful in case of sterile samples like CSF pleural fluid etc where no. of pus cells and bacteria will be less