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it produces a brick/dark red stain.
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∙ 13y ago
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Why distilled water is tested with Sudan IV in experiment of test for lipid?
because it want to show that Sudan iv is soluble in water n not soluble when there is lipid presence . From there you can make lipid as your responding variable
What chemical property allows lipids and Sudan IV dye to mix?
The sudan dye is a lipid itself, therefore you have a lipid layer interacting with another lipid.
How Sudan IV detect the presence of lipid?
The chemical Sudan IV is not soluble in water; it is, however, soluble in lipids. Therefore to test for the presence of lipids in a solution you will use a Sudan IV Test. In this test dark red Sudan IV is added to a solution along with ethanol to dissolve any possible lipids. If lipids are present the Sudan IV will stain them reddish-orange, giving a positive test.
How do you use the Sudan IV test?
Sudan IV is a diazo dye for staining lipids, lipoproteins, and triglycerides. It is used in Sudan staining, wherein it attaches to the fats chemically.
What is the limitation for the lipid test?
Sudan IV is only soluble in certain solvents.
What is a positive Sudan IV test result?
If no lipids are present, the red dye will sink to the bottom of the test tube. Sudan (iv) is not soluble in water but soluble in lipid.
What color is Sudan iv?
I think its red....
What is the difference between Sudan IV Lipid Test and Translucence Lipids Test?
We just did a lab on this in my bio class.The Sudan test uses Sudan IV reagent. If a lipid or fat is present, red globs of fat will appear at the top of the solution. If not, there won't be a reaction.The translucence test is when a drop of liquid is placed on an unglazed paper. Water will dry up and leave the paper crinkly. An oil or fat will spread out, and leave a stain, not really drying, and making the paper look translucent.
Importance of Sudan IV?
Like lipids, the chemical Sudan IV is not soluble in water; it is, however, soluble in lipids. Therefore to test for the presence of lipids in a solution you will use a Sudan IV Test. In this test dark red Sudan IV is added to a solution along with ethanol to dissolve any possible lipids. If lipids are present the Sudan IV will stain them reddish-orange, giving a positive test.
Why does lipids turn redish brown when added to Sudan iv dye?
Sudan dye is lipid -soluble dye, thus, it will turn any lipids to red. It mixes with it.
Why is there a color change when Sudan iv is added to a fat?
There is a colour change because like fats, Sudan IV is not soluble in water. So when you add Sudan IV to lipids, it will break down therefore the chamical reaction that takes place is the colour change.
Which test uncoated paper test or Sudan test is the most sensitive to small quantities of lipids?
for me, i think the uncoated paper test is more sensitive than Sudan iv test. This is because Sudan iv test can only soluble in certain solvent thus make it harder to define either the solution that is tested is really have lipid in it although there is not. The uncoated paper test can show if there is lipid the the solution if greasy transparent spot comes out from the uncoated paper.
