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What else can I help you with?
What considerations need to be made when choosing an elution buffer the mobile phase in a chromatographic run?
You need to consider the pH of the elution buffer of the mobile phase in a chromatographic run. You should work within 1 pH unit of the buffer pKa value.
Why is tris buffer made up fresh?
Tris buffer should be made fresh because over time, tris can hydrolyze and form acidic impurities, leading to a pH shift in the buffer. This can affect the accuracy and reliability of experimental results when using the buffer in biological or biochemical assays. Making the buffer fresh ensures that its pH and composition are reliable and consistent.
Is Glucose a good buffer?
No, glucose is not a good buffer. Buffers are made of weak acids and their conjugate bases or weak bases and their conjugate acids. Glucose does not act as a buffer because it is a simple sugar and does not have the necessary acidic or basic properties.
What is a good sentence for the word buffer state?
After World War 1, attempts were made between Germany and France to make buffer states.
What is the difference between a concentrated buffer and a diluted buffer?
A concentrated buffer has a higher concentration of buffer components compared to a diluted buffer. Concentrated buffers are typically used for preparing stock solutions, while diluted buffers are used for specific applications where a lower concentration is needed. Diluted buffers are often made by diluting a concentrated buffer with water or another solvent.
Is KCl plus NaCl a buffer system?
No, a buffer system is made up of a weak acid and its conjugate base or a weak base and its conjugate acid. KCl and NaCl are both strong electrolytes and do not act as a buffer system when combined.
Where can you get a bolt buffer for a .22 long BAR Browning rifle?
After much research, a manufacturer could not be found that made a buffer for your model. Perhaps a request to Browning would be in order. Try a Buffer manufacturer and request that they make one for your .22 BAR.
What is the pH of Triethylammonium acetate buffer?
The pH of a buffer solution containing triethylammonium acetate would depend on the concentration of the components. Typically, a buffer solution made from triethylammonium acetate and acetic acid would have a slightly acidic pH, around 4 to 5.5.
Compare the buffer system in the whole blood?
The buffer system in whole blood is made up of carbonic acid-bicarbonate buffer system and protein buffer system. The carbonic acid-bicarbonate buffer system helps regulate pH by balancing the levels of carbonic acid and bicarbonate ions. The protein buffer system involves proteins like hemoglobin that can bind to and release hydrogen ions to help maintain a stable pH in the blood.
Two components that make up a buffer include?
A buffer is made up of an acid and its conjugate base.
What is the chemical substance that resists change in pH?
A buffer solution is a chemical substance that resists changes in pH when small amounts of acid or base are added. Buffers are made up of a weak acid and its conjugate base, or a weak base and its conjugate acid, which work together to maintain the pH of a solution.
When Dr. Farrell was a graduate student he once made up a pH 8.0 sodium acetate buffer. Why would the casual observer to this buffering faux pas come to the conclusion that he?
Not exactly sure what the question is asking, but sodium acetate will not buffer at pH 8. It is an ok buffer in the pH range of maybe 3-5 or so. Acetic acid is the weak acid of this buffer with a pKa near 10^-5. To make a decent buffer at pH 8, one needs a weak base, or a weak acid with a pKa closer to 8.
