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Due to their nature obligate intracellular parasites viruses cannot be grown in standard culture media, they must be grown on living cells or in multi- celled organisms. Various methods by which host cells may be maintained to support virus growth include-

1) Solid mediums - plaque assay-mixing cells and virus using pour plate or soft agar overlay method.

2) Growth in liquid culture- by mixing together host and viral cells

3) Growth in tissue culture - cells from plants or animals may be grown 'in vitro' often in flasks on a plastic substrate

4) grown in host organism -viruses which normally infect plants or animals or bacteria can be grown simply by infecting the living host

5) Embryonated eggs - fertilized and growing eggs usually from chickens make an excellent cheap 'in situ' growth chambers

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12y ago

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