There are several things that determine an enzyme's activity. The main determinants include the structure of the enzyme, temperature, pH and so much more.
An enzyme shape determines it's activity.
Temperture
Phosphorylated enzymes may be more or less active than non-phosphorylated enzymes
Enzymes generally work within a given temperature range. As the temperature is increased the activity will increase. However if the optimum temperature is surpassed, the enzymes will stop working.
Enzymes are very pH-sensitive. Any change in pH results in denaturing of proteins.
Enzymes activity is affected by temperature. At a very high temperature, enzymes became denature that means they lose their original shape, which is important for them to react. Thus, enzyme activity decreases at a very high temperature.
One, by the genetically controlled 'copy number' of the number of individual enzymes available to the cytoplasm; two, by the concentration {or presence} of control factors that determine the activity of individual enzymes; and three, by the presence or absence of various termination factors that determine which Version of an enzyme is to be produced.
It is because extracellular enzymes act under very hostile conditions in nature, so their activity is more resistant than intracellular activity.
what i know is enzymes are denatured in organic solvents loosing their activity.
Temperture
lysosomal enzymes
Temperature
The activity of the enzymes depends on the specific pH needed.
Shapes
Temperature, pH, substrate concentration
Enzymes are derived from polypeptide chains, enzymes are responsible for adequate cell activity. Therefore, if a polypeptide chain is not properly composed, the enzyme will be dysfunctional, hence leading to a depletion in cell activity.
Phosphorylated enzymes may be more or less active than non-phosphorylated enzymes
Boiling usually destroys enzymes.