Phosphorylated enzymes may be more or less active than non-phosphorylated enzymes
The pH is varied to effect, by its affect, this test.
At low concentration of substrate , rate of enzyme action is directly proportional to conc. of substrate .
Different enzymes work best at different pH. This is refered to as the ideal pH for the enzyme. For example, the digestive enzyme trypsin works best at an acidic pH while alkaline phosphatase works best at a basic pH. Therefore, enzyme activity varies with pH and this variation depends on the enzyme being studied
activators; inhibitors
Hexokinase is an enzyme involved in the phosphorylation of hexose (five carbon sugar). It speeds the process on adding a phosphorus to the sugar.
Yes. Hormones may stimulate the reversible covalent modification of an enzyme via phosphorylation or dephosphorylation and alter the activity of the molecule.
Denature enzyme activity
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
Inorganic phosphate can be used as an inhibitor under covalent modification. Phosphorylation/ Dephosphorylation can either activate or deactive an enzyme.
Activation, conversion from glycogen phosphorylase B to glycogen phosphorylase A
pH
glucokinase
Creatine KINASE
a hexokinase is an enzyme that catalyzes the phosphorylation of hexoses.
The pH is varied to effect, by its affect, this test.
temperature and pH
At low concentration of substrate , rate of enzyme action is directly proportional to conc. of substrate .