temperature and pH
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
It is because extracellular enzymes act under very hostile conditions in nature, so their activity is more resistant than intracellular activity.
The rate of a reactions usually increases when catalyzed by an enzyme. For maximum rate of activity, the enzyme needs optimal conditions.
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
The activity of an enzyme is affected by temperature, pH and the concentration of the substrate.
Denature enzyme activity
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
pH
The enzyme activity depends on the conditions of the reaction and the enzmye used. The term enzyme activity refers to the rate at which substrate is converted into product and can be measured in many different ways.
The pH is varied to effect, by its affect, this test.
It is because extracellular enzymes act under very hostile conditions in nature, so their activity is more resistant than intracellular activity.
Low levels of ATP
The rate of a reactions usually increases when catalyzed by an enzyme. For maximum rate of activity, the enzyme needs optimal conditions.
temperature,pH and substrate concentration
pH Temperature Substrate Concentration non-ideal conditions will ultimately lead to the denaturing of the enzyme
At low concentration of substrate , rate of enzyme action is directly proportional to conc. of substrate .
many vitamins and minerals play crucial roles in many metabolisms as coenzyme or cofactor. Deficiency of those lower the related-enzyme activity.