0
NCBI Blast is an acronym for Basic Local Alignment Search Tool. It is used to find areas of local similarity between protein sequences. It is used to decide if there is any statistical significance between matches.
Wiki User
∙ 11y ago
Add your answer:
Earn +20 pts
Why you use NCBI database in bioinformatics?
The NCBI hosts many databases that are very useful to bioinformaticians, including genetic sequences, genetic variants and literature.
Why do RefSeq accession numbers for mRNA sequences start with the letters NM in particular?
It is the reference sequence (as opposed to a genbank sequence) for an mRNA. It has been curated by the nice people at NCBI to act as a baseline for the scientific community. See http://www.ncbi.nlm.nih.gov/projects/RefSeq/RSfaq.html for further explanation.
What is Mendalian inheritance and how does it work?
Mendelian Inheritance in Man (MIM) is a database that catalogs all the known diseases with a genetic component, and-when possible-links them to the relevant genes in the human genome. MIM is one of the databases housed in the U.S. National Center for Biotechnology Information (NCBI) and included in its search menus. It was started in the early 1960s and is available as a book currently in its 12th edition. The online version, OMIM, has been available since 1987, moved to the world wide web by NCBI in 1995, and in 2004 contained more than 15,000 records.Source: Wikipedia
Where can you find the genomic and transcript sequences of the gene PKD1 and the amino acid sequence of the protein polycystin-1?
Try the NCBI (National Center for Biotechnology Information) site. On the home page (there is a link below) enter PKD1 in the search box.A good website for human (and other) gene locations and sequences is Ensembl. Try, for example, clicking the link below, and use the left side-bar, or scroll down for the transcript sequence.All information on the Ensembl website is in the public domain.There is relevant discussion in Cell 1995 Apr 21; 81(2):289-9. See the Cell Press link below for a PDF of the Cell article.
How can I check am I using correct primer sequence in my PCR?
you should use "Primer blast" at NCBI site
What is NCBI?
NCBI is a primary database . it stand for national center for biotechnology information.
Why you use NCBI database in bioinformatics?
The NCBI hosts many databases that are very useful to bioinformaticians, including genetic sequences, genetic variants and literature.
What kind of information can someone take from NCBI?
The National Center for Biotechnology Information, or the NCBI, is a branch of the United States National Library of Medicine, or the NLM. With access to the NCBI, one can't acquire very much unless one has a patient's medical information.
What kind of research does NCBI conduct?
NCBI (The National Center for Biotechnology Information) conducts scientific research. Specifically, they do research on genomic and biomedical information to advance our knowledge in health and science.
What is the Scientific name for Cynaobacteria?
Here is a good place to start looking: http://www.bacterio.cict.fr/classifcyano.html#ncbi
How many bacteria strains or substrains have been recorded in NCBI taxonomic classification?
Bacteria Taxonomy total # 184,921
How do you find genes unique to candida albicans using bioinformatics?
By exploring in biological databases such as NCBI you can find the specific genes of any organism. By aligning the genes of Candida with related species of bacteria or fungus one can fish out the unique genes of Candida that are not common in other organism. There are tools for these sequence alignments (eg:BLAST, FASTA)
What next steps after sequencing DNA sample?
You'll want to clean your vector sequence off of your cloned sequence first. There is a program called vec screen that is free online. If it is multiple sequences you can use a variety of software programs. DNAbaser is free and for windows. If you keep searching the web you'll find a good opensource program. For mac users try Sequencher. this is by far the best program out there for sequence cleanup as a batch run.You will then want to trim ambiguities off, which are all the N's or undetermined base calls. You can also view your chromatogram and tweak it, but my god, will that bore you and waste time...unless you have time to waste.Next you will want to find what your sequence is most similar to in the public databases.For EST and cDNA library sequences you'll want to do a batch blast using a software program such as Blast2Go (a java app.). For a single sequence you will want to just use the NCBI Blast web page and choose if it's human, mouse, plant, etc.You can search for protein similarities using Blastx or nucleotide similarities using Blastn. Other options exist and the NCBI site has a whole tutorial for you. Have fun.
What computer skills needed by a biotechnology student?
As far as technical skills are concerned, there are not many. A through knowledge of MS office is required. Get familiar with plotting different types of graphs and alternative ways of representing data with MS excel. There is a statistical data analysis software called SPSS (version 16 or higher). Learn how to use it. There are several online tutorial available. Learn how to use to the free sequence analysis tools available on the NCBI website. Examples include BLAST, Virtual PCR and Primer design. If you are interested in Bioinformatics, learn the following programming languages: # PERL # R
Where can one find information about sepsis?
One could find information about sepsis or blood poisoning online at various websites. The most recommended websites are 'Wikipedia', 'Mayoclinic' and 'NCBI'.
How many visually impaired people are there in Ireland?
It's a hard one to put a figure on however the NCBI (National Council for the Blind) lists 14,000 people using their services.
