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You'll want to clean your vector sequence off of your cloned sequence first. There is a program called vec screen that is free online. If it is multiple sequences you can use a variety of software programs. DNAbaser is free and for windows. If you keep searching the web you'll find a good opensource program. For mac users try Sequencher. this is by far the best program out there for sequence cleanup as a batch run.
You will then want to trim ambiguities off, which are all the N's or undetermined base calls. You can also view your chromatogram and tweak it, but my god, will that bore you and waste time...unless you have time to waste.
Next you will want to find what your sequence is most similar to in the public databases.
For EST and cDNA library sequences you'll want to do a batch blast using a software program such as Blast2Go (a java app.). For a single sequence you will want to just use the NCBI Blast web page and choose if it's human, mouse, plant, etc.
You can search for protein similarities using Blastx or nucleotide similarities using Blastn. Other options exist and the NCBI site has a whole tutorial for you. Have fun.
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Why are ddNTPs used for DNA sequencing?
Because on the addition of the normal ddNTP to the sequencing, there will be addition of another nucleotide added by DNA polymerase which keeps DNA sequencing going.
Who discovered the technique of DNA sequencing?
DNA sequencing was first discovered by Fredrick sanger in 1950s
What are the steps in a direct-sequencing experiment?
Lyse cells, purify DNA, amplify genes by PCR, and insert genes into plasmid
Describe what is meant by the term rapid sequencing?
Sequencing DNA rapidly
What process could a what forensic scientist use to analyze a DNA sample from a crime scene to identify a criminal?
A forensic scientist would use a method like PCR (Polymerase Chain Reaction) to amplify the DNA sample, creating more copies for analysis. They would then use DNA sequencing techniques to compare the sample to a suspect's DNA profile or a DNA database to identify a match. This process helps determine the source of the DNA sample and link a suspect to the crime scene.
Dideoxyribonucleotide chain-termination is a method of?
Sequencing DNA (Sanger Sequencing Method).
What does not read DNA sequences?
People not versed in DNA sequencing.
What is the concept of genetic sequencing about?
When looking for information about the sequence of DNA then there is information relating to the concept of genetic sequencing available from Wikipedia. The site offers about DNA sequencing with links that relate to other facts and information on the different aspects of genetic sequencing.
What is meant by pyrosequencing?
It is common knowledge that pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle.
Why would a person use Sanger sequencing?
Sangers sequencing technique is used to determine the order of nucleotides in a strand of DNA (deoxyribonucleic acid). It was first used to discover the order of the nucleotides in the genomes of various organisms during the Human Genome Project.
How is DNA tested and why?
by DNA fingerprinting method , DNA-DNA hybirdization or DNA sequencing. to know the sequence of DNA
What does guanine combines with?
In DNA sequencing, Adenine and Guanine are known as "base pairs", and are purines, which form the building blocks of DNA and RNA. Guanine combines with Adenine in DNA sequencing.
