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What does Thioglycollate contribute to culture media?

Updated: 8/19/2019
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Q: What does Thioglycollate contribute to culture media?
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What does a blue or pink color in thioglycollate mean?

If its Fluid thioglycollate media - a red colour indicates thatg the media has been oxygenated - which is not desirable. The colour change is due to a chemical called resazurin in the media.


What type of media is used to demonstrate oxygen requirements of microbes?

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Why is sodium thioglycollate used in lab media?

It is used as a reducing media. Thus minimizing the level of oxygen in the media.


Why are cysteine and sodium sulfide added to the growth media?

To culture obligate anaerobes, all molecular oxygen must be removed and kept out of the medium. Addition of oxygen-binding agents such as thioglycollate, the amino acid cysteine, or sodium sulfide to the medium prevents oxygen from killing the anaerobes you want to culture.


Why can media such as brain-heart infusion and thioglycollate be used for the cultivation of anaerobes?

Anaerobes cannot be cultivated in the presence of atmospheric oxygen.


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The properties and purposes of thioglycollate medium?

A thioglycollate medium is used for culturing anaerobic bacteria.


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