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The concentration of hydrogen ions in solution affects the enzyme activity. Each enzyme has maximal efficiency under an optimum pH.
Since pH is one of the factors for the denaturation of proteins, if an enzyme is submitted to a pH level under which it is denatured there will be no enzymatic activity.
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What can you do to regain the activity of the enzyme?
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
What are the effects of freezing on enzyme activity?
When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.
What is the effect of cold temperatures on enzyme activity?
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
What blocks enzyme activity by binding to allosteric site of an enzyme causing the enzyme's active site to change shape?
An allosteric inhibitor stops enzyme activity by binding to an allosteric site and causing the conformation of the enzyme to change.
What affect does too much substrate have on the rate of an enzyme reaction?
The enzyme becomes saturated by the substrate and enzyme activity plateaus.
What can you do to regain the activity of the enzyme?
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
What is the independent variable between temperature and enzyme activity?
To find out how enzyme concentration affects the activity of the enzyme you must:vary the concentration of the enzyme, by preparing different concentrations (keeping the volume of solution the same)keep the temperature, substrate concentration and pH constantmeasure the activity of the enzyme at each concentrationHow the enzyme activity is measured will depend on the specific enzyme involved.You need to have plenty of substrate (excess substrate) so it doesn't run out during the experiment.In this type of experiment, the enzyme activity is the dependent variable, the temperature, pH and substrate concentration are control variables and the enzyme concentration is the independent variable.
What is the effect of hydrochloric acid on the enzyme activity?
Denature enzyme activity
What are the effects of physical activity on enzyme activity?
Physical activity can alter the shape of enzyme which can cause damage or may the enzyme become inactive
What are the effects of freezing on enzyme activity?
When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.
What switchs on enzyme activity while what can switch off or reduce enzyme activity?
activators; inhibitors
What is the effect of cold temperatures on enzyme activity?
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
What can change the way an enzyme acts?
Enzyme activity is affected by other molecules, temperature, chemical environment (e.g., pH), and the concentration of substrate and enzyme. Activators are molecules that encourage enzyme activity, and inhibitors are enzymes that decrease enzyme activity. Sometimes a cofactor is necessary for the enzyme to work.
Which blocks enzyme activity by binding to the site of an enzyme?
inhibitor
What accurately describes how temperature affects enzyme activity?
Enzyme activity increases with temperature, but only up to a point.
What determine an enzymes activity?
There are several things that determine an enzyme's activity. The main determinants include the structure of the enzyme, temperature, pH and so much more.
Why is protein concentration necessary for accurate comparison of the enzyme activity of the fractions?
Enzyme activity sometimes reflects the amount of protein expressed in a cell--however, due to enzyme inhibitors, the enzyme activity is not always reflective of the amount of protein expressed by a cell.
