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This is what you do when you want to see all of the pigments that make up one color. You can do this at home with a coffee filter, and a non-permenant marker. Rip the coffee filter into strips and put a little dot with the marker at the end of one of the strips. Then place the end with the mark into a really shallow cup of water and allow the filter to absorb it. It may take some time but the pigments will be lifted by the water, and it will look like a rainbow!
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What is a chromtography?
Hi is it got to do with Science? Its Chromatography .. Actually right now I'm studying Chromatography it's a really interesting unit. It is a way of seeing the other different colours that are mixed together to make that colour. As an experiment you have filter paper and because it has very small holes that you cannot see with a naked eye, liquids are most likely to be the only ones passing through. Anyway, anything that has to do with colour e.g food colouring, permanent marker etc... the filter paper will absorb the ink and will spread make sure the ink is about 2cm up your paper. You then dip the filter paper into a beaker of water - only about a cm full of water. After a few minutes the ink will naturally spread and you will be able to identify what other colours are used to create the colour of your chosen type of ink or whatever. They use Chromatography for like police investigations with DNA and that sort of stuff ...
Is ion-exchange chromatography is reverse phase chromatography?
The principal of ion exchange chromatography is the most popular method for purifying protein compounds.Other charged molecules are also called an ion exchange if of a protein nature.[Fig 1. Principle of anionic and cationic exchangers.]Biomolecular binding strength depends on solution pH, since it affects the number of ions available for exchange. Proteins can be zwitterions, so you'll need to use either an anionic or cationic exchanger. Whatever the conditions, we have to determine the isoelectric point of the protein at different pHs to see what charges the proteins can have and where it's electroneutral. When pH < pI of a given molecule, it will be positively charged and we'll need a cation exchanger; vice versa when pH > pI. The amphoteric character (ability to react as either acid or base) of proteins allows us to detect ionic interference of other substances and improve our own protein separation. The principle of ion exchange processes will be explained more clearly using an anionic exchange as an example. Today, most people use synthetic organic ion exchangers on a polystyrene base (DOWEX) or natural polymers like cellulose, dextran, or silicate. The macromolecules of the ion exchanger normally make up a 3D network, onto whose surface a huge number of ionizable groups are covalently bonded. Whereas the type of matrix material is generally flow characteristic (the type of ions used and their chemical/mechanical stability are more solid), the groups covalently-bonded to the matrix and the strength of those bonds determine what the exchangable ions can be: every group gives an exchange of very basic (anion exchanger) for very acidic (cation exchanger) character.[Fig 2. Principle of an ion exchange process.]Typical functional groups in an anion exchanger are quaternary amines such as diethyl aminoethyl groups (DEAE - non-denaturing, sorbents have good loading capacity), while those for cation exchangers include organic and inorganic acids like carboxymethyl groups (CMs) or sulfonates. These groups are covalently coupled to the matrix material (Fig. 3). Since exchange groups are only inserted in their ionic form, it's important to know their pK values. Such values can be found by a simple titration curve, as shown for a CM sephadex in Fig 4. Because so many biologically important substances contain ionizable functional groups (amino acids, proteins, nucleotides, nucleic acids, metabolites, etc), biochemical methods for the isolation and separation of charged compounds are quite valuable - some charged compounds are electrostatically bound to the exchanger and others are not.
