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What does tetracyclin do?

Updated: 8/16/2019
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14y ago

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It is a broad spectrum antibiotic that is particularly successful in treating Rickettsial infections.

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14y ago
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Q: What does tetracyclin do?
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What is the definition of competent cells and how it can help to make a transformation more efficient during the cloning of desired gene?

Generally most of the bacteria have the ability to take up DNA which is present freely in the environment. This is called Competence. It is genetically acquired. But some cells do not exhibit natural competence and they are treated with a suitable chemical like CaCl2 to make their cell wall relatively permeable to DNA. We can make any cell competent in vitro and introduce our desired gene which is taken up by the competent cell. To test the efficiency of our treatment, we may introduce a marker gene like gene for antibiotic resistance such as Tetracyclin onto the desired gene and grow the treated cells on Tetracyclin medium so that only the cells that have taken up the DNA survive.


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