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There are a variety of enzymes used in replication. Helicase is used to open the hydrogen bonds that connect the two strands. However, this causes a tension to form in the strands (like a wind up toy) so some of it needs to be released. This is done by topoisomerase, which cuts the strands, lets them spin out some of the tension and attaches the DNA back together again. Moving behind helicase, is an enzyme called SSBP. This basically binds to the DNA sequence to prevent it from reattaching to itself after helicase unzips it; DNA would otherwise just bond back with the other strand. Then an RNA Polymerase called primase comes and attaches a primer to the DNA strands. This is needed because the next enzyme, DNA polymerase will not from scratch and needs a base to work from: the primer serves this role. Starting on the primer, DNA Polymerase III synthesizes the new strand, but the primers are still left on the strands. These will be removed by DNA Polymerase I which also adds new nucleotides to the hole left by the primer. Finally, an enzyme called ligase fills the one nucleotide gap left between the primer and the newly synthesized DNA with a sugar phosphate backbone (not another nucleotide)

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12y ago
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13y ago

DNA Helicase - The enzyme responsible for separating the two strands of DNA, normally in the conformation of a helix, so that they can be copied during DNA replication.

DNA Ligase - The enzyme responsible for sealing together breaks or nicks in a DNA strand, and responsible for patching together Okazaki fragments on the lagging strand during DNA replication.

DNA Polymerase - The enzyme responsible for catalyzing the addition of nucleotide subunits to DNA both during and after DNA replication.

Primase - The enzyme responsible for initiating synthesis of RNA primers on the lagging strand during DNA replication.

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12y ago

Helicase- enzyme that helps in unwinding the DNA molecule

DNA polymerase- adds the complementary base pairs

DNA replication is a semi-conservative process. This means that after a strand of DNA is replicated, half is old and half is new.

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13y ago

DNA Polymerases-

They bond nucleotides (C,G,A,andT) together to make a new strand in order.

Another enzyme is DNA Ligase-

They seal the gaps in a new DNA strand.

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13y ago

Helicase, and DNA polymerase .

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12y ago

mRNA and tRNA

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Q: What nucleic acids are involved in translation?
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