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What gene makes it possible to destinguish bacteria that have been transformed by a plasmid?
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How is it possible to splice foreign DNA into a plasmid without altering the bacteria's ability to function normally?
A plasmid is an extra-chromosomal element. Strictly speaking, it is not a part of the bacterial mail genome. Plasmids, when first discovered in bacteria, were observed to contain genes that confer special features to the bacteria (like drug resistance). Also, a plasmid is capable of autonomous replication, which means it has its own origin of replication and does not have to wait for the bacterial genome to be replicated. When a foreign gene is inserted into a plasmid, the plasmid has to be transformed into the bacteria. Once transformed, the cDNA in the plasmid is able to replicate every time the plasmid replicated. This does not have any affect on the normal functioning of the bacteria. The organism is merely being used to make copes of the foreign gene and/or harvest an important protein product.
A plasmid a foreign gene and recombinant DNA would be associated with what type of organism?
A plasmid is a double stranded cirucular DNA, used as a vector in cloning. A gene of interest can be ligated into the this to form a chimeric DNA or rDNA. This can be transformed to a bacteria for propagation of the clones (you can amplify them by these transformed bacteria).
What gene makes it possible to identify bacteria that carry a plasmid?
a genetic marker
A gene that makes it possible to distinguish bacteria that carry the plasmid from those that dont?
Genetic marker.
What enzyme is produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18?
Luciferase
A bacterial cell that has taken up plasmid DNA is?
A bacterial cell that has taken up plasmid DNA is transformed.
What organisms have plasmid?
Bacteria
What is col plasmid?
A plasmid (free bit of DNA) in bacteria that produces colicin to kill other strains of bacteria.
Why does bacteria that contains plasmid glow in uv light?
the plasmid contains a certain gene, which codes for the "Green Flourescent Protein." So you put the plasmid in the bacteria, the plasmid starts making that protein in the bacteria, and boom you've got glowing bacteria. works for bunnies and monkeys too, apparently =)
Does a bacteria cell have a plasmid?
Yes
What are plasmid libraries?
A plasmid is a piece of circular DNA. These plasmids are transformed into bacteria/yeast. A library is made by taking pieces of DNA (from a genomic DNA digest, or cDNA) and inserting them into plasmids. The plasmids are then transformed into the organism and stored. Libraries are used to screen for new functional genes, usually when looking at new substrates or products that the yeast/bacteria can't make without the plasmid.
What is one way to determine whether a bacterial culture has received a recombinant plasmid?
The plasmid have a "reporter gene" inside it, generally resistance to specific antibiotic. the plasmid is transformed into bacteria that don't have resistance to that specific antibiotic drug, and than the cultured on a petri-dish that contain the antibiotic drug. Only bacteria that had receive the plasmid will have resistance and grow, all the other will die.
