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The restriction enzymes have ability to cut on a specific sequences that are knwon as palandreomic sequences now they cut in two way i.e. blunt and sticky
for e.g. if we have a sequece of ATTCGTAAGC it will be cutted in two way as
...................................................TAAGCATTCG
1. ATTCG......... TAAGC i.e., blunt or straight form all ends
....TAAGC .........ATTCG
2. ATT .................CGTAAGC
....TAAGCAT .................TCG i.e., sticky or uneven ends
The reason for this phenomena is still not clear or knwon
Wiki User
∙ 14y ago
Wiki User
∙ 16y agoNo restriction enzymes are capable of producing both sticky and blunt ends simultaneously,either they create sticky or blunt ends . Hence there is no question of joining them.
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What is the restriction site of the restriction enzyme Hae III?
The restriction site of Hae III is GGCC. It cuts between the G and the C. This produces blunt ends.
What is the restriction site to restriction enzyme Hae III?
Hae III cuts at the site GGCC. It creates blunt ends - meaning a clean cut. This is found between the G and C.
What is a restiction enzyme?
A restriction enzyme is used to cut DNA (either double or single stranded), cuts can either be "sticky" ( in which cut pieces overlap one another), or "blunt" (in which cut pieces do not overlap). A common rectriction enzyme is EcoR1.
What is Example of restriction enzyme?
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:5'-- GAATTC --3'3'-- CTTAAG --5'After this DNA sequence is cut, it might look something like this:5'-- G AATTC --3'3'-- C TTAAG --5'
How can a restriction enzyme leave sticky ends between DNA fragments?
The sticky ends generated by restriction enzymes can easily be joined using an enzyme called ligase. Blunt ends however, cannot be joined so easily. This is why restiction enzymes that create sticky ends are more useful. If blunt ends result, small segments called modifiers are attached to the sticky ends. These modifiers are nucleotide sequences that have sticky ends and attach to the blunt ends, thus making them sticky ends.
What is the restriction site of the restriction enzyme Hae III?
The restriction site of Hae III is GGCC. It cuts between the G and the C. This produces blunt ends.
What is the restriction site to restriction enzyme Hae III?
Hae III cuts at the site GGCC. It creates blunt ends - meaning a clean cut. This is found between the G and C.
What is a restiction enzyme?
A restriction enzyme is used to cut DNA (either double or single stranded), cuts can either be "sticky" ( in which cut pieces overlap one another), or "blunt" (in which cut pieces do not overlap). A common rectriction enzyme is EcoR1.
What is Example of restriction enzyme?
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:5'-- GAATTC --3'3'-- CTTAAG --5'After this DNA sequence is cut, it might look something like this:5'-- G AATTC --3'3'-- C TTAAG --5'
What seals the sticky ends of restriction fragments to make recombinant DNA?
These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.
How can a restriction enzyme leave sticky ends between DNA fragments?
The sticky ends generated by restriction enzymes can easily be joined using an enzyme called ligase. Blunt ends however, cannot be joined so easily. This is why restiction enzymes that create sticky ends are more useful. If blunt ends result, small segments called modifiers are attached to the sticky ends. These modifiers are nucleotide sequences that have sticky ends and attach to the blunt ends, thus making them sticky ends.
What are three ways that people use restriction enzyme?
Restriction enzymes (REN's) cut or cleve DNA. This cutting process is sequence-specific. Which means, the enzyme does not randomly fragment the DNA (or template strand as it is generally called in many experimental protocols). Instead, the enzyme scans the length of the template until it reaches a specific sequence of 4 - 8 nucleotides. Once this sequence (the restriction site) is reached, the enzyme produces a cut in the template and generates either blunt ends (no overhangs) or sticky ends (having both 3' and 5' overhangs) The primary application of restriction enzymes is is genetic engineering where we want to insert cDNA sequences into plasmids or other vectors.
Does blunt wraps have nicotine?
Yes. Blunt Wraps are accually compressed Tobacco Pulp.
What happens if you eat a blunt?
You can face gastric problems.
Function of restriction enzymes?
They cut strands of DNA at specific sites.
What are linkers in genetic engineering?
these are short pieces of double stranded DNA of known nucleotide sequence,its a blunt-ended,but contains a restriction site.
When you smoke a blunt you get a headache why?
The most likely reason is that it's the nicotine headrush from the blunt wrap. These are made from tobacco leaves, and if you aren't used to smoking tobacco, it can give you a headache.
