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What is the counter or secondary stain used in the Gram stain procedure?

The counter or secondary stain used in the Gram stain procedure is safranin.


Does yogurt stain gram positive or gram negative?

Yogurt contains mostly lactic acid bacteria, which are Gram-positive bacteria. This means they will stain purple under a Gram stain.


What is the difference between gram-positive and gram-negative bacteria?

The presence of thick cell wall. Gram positives have a thick cell wall and remain blue. Gram negatives have a thin cell wall where the blue stain washes out and then will stain pink with the counter stain.


Does candida have a positive or negative gram stain?

Gram positive


What is the gram stain for Epidemic MRSA?

Gram Positive.


What gram stain is scarlet fever?

gram positive


Is HIV gram stain positive or negative?

The answer to whether HIV gram-stain positive or negative is that HIV gram-stain is negative. They retain the light red or pink color after the stain.


What does gram negative on a serratia marcescens gram stain mean?

Bacteria are gram positive or gram negative. Serratia happens to be a gram negative bacteria. They appear pink on a gram stain. Gram positive bacteria stain to a purple color on a gram stain. We can classify and ID bacteria using their gram stain and shape. Some antibiotics only work on gram negative bacteria and some only work on gram positive bacteria. It helps a doctor know which antibiotic to use.


Why safranin is used in counter stain?

Safranin is used as a counterstain in Gram staining to colorize Gram-negative bacteria, as they do not retain the crystal violet primary stain. This allows for better contrast and differentiation of Gram-negative bacteria against the purple Gram-positive bacteria.


Is B subtilis a gram positive or gram negative bacterium?

gram positive Exactly. When doing a gram stain on B. subtilis, this bacterium resists decolorization (keping the first stain and NOT taking on the color of the secondary stain). Therefore, this bacterium is gram (+).


What is gram positive strain?

Gram-positive bacterium are those that are stained dark blue or violet by Gram Staining. This is in contrast to Gram-Negative Bacterium, which cannot retain the crystal violet stain, instead taking up the counter-stain and appearing red or pink. Gram-positive organisms are able to retain the crystal violet stain because of the high amount of peptidoglycan in the cell wall. Gram-positive cell walls typically lack the outer membrane found in Gram-negative bacteria.


What is the gram stain for clostridium perfringens?

Clostridium perfringens is a Gram-positive bacterium, meaning it will stain purple/blue with the Gram stain procedure due to its thick peptidoglycan layer.