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The Persian war was their worst war
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Diesel-Electric submarines had been in use for years before WWI; there were many commissioned boats on both sides of the war before it even started, so there is no one particular submarine used in WWI. However, the one boat that arguably had the most impact at the beginning of WWI was the German submarine U-20, which was responsible for sinking the British passenger liner RMS Lusitania. This single submarine attack had repercussions throughout the war, causing policy shifts in Germany considering submarine warfare, and which affected submarine warfare in general forever.
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