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Polymerase chain reaction
Polymerase Chain Reaction
PCR stands for Polymerase Chain Reaction, a method used to amplify and copy small segments of DNA.
The polymerase used in polymerase chain reaction (PCR) is typically derived from a thermophilic bacterium called Thermus aquaticus. The specific polymerase most commonly used is Taq polymerase, which is known for its ability to withstand high temperatures required for PCR.
polymerase chain reaction
In addition to DNA polymerase and primers, a polymerase chain reaction (PCR) requires deoxynucleotide triphosphates (dNTPs), which are the building blocks of DNA, and a buffer solution to provide the optimal pH and ionic environment for the reaction. Additionally, a thermal cycler is needed to facilitate the precise temperature changes required for denaturation, annealing, and extension during the amplification process.
To bring about a polymerase chain reaction DNA sequences are placed in .2-.5ml reaction tubes and then placed in a thermal cycler. To achieve the reaction the sequences must undergo 20-40 temperature changes.
polymerase chain reaction
Polymerase chain reaction
Polymerase chain reaction
Polymerase chain reaction
Polymerase chain reaction