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What has the author RAJA GHOSH written?
RAJA GHOSH has written: 'PROTEIN BIOSEPARATION USING ULTRAFILTRATION: THEORY, APPLICATIONS AND NEW DEVELOPMENTS'
What are scope and state of art of bioseparation?
Bioseparation refers to the process of separating biomolecules in a mixture. It is a crucial step in various fields such as biotechnology, pharmaceuticals, and food processing. The state of the art in bioseparation includes advanced techniques such as chromatography, electrophoresis, and membrane filtration, which enable high-efficiency separation of biomolecules based on their specific properties. These techniques are constantly evolving to enhance separation efficiency, resolution, and scalability for various applications in the biotechnology industry.
Which areas of research does Department of Bioprocess offered?
"Currently, the research areas offered by our department fall into the following fields: - Fermentation Technology & Bioreactor Design - Molecular Biology & Genetic Engineering - Bioseparation - Tissue Culture Engineering - Food Engineering - Biosensor - Biosensor"
What are the basic principles of Bioprocess?
bioprocess engineering principles include genetic engineering, metabolic engineering enzyme technology fermentation chemical engineering nanotechnology biomedical engineering molecular biology biochemistry microbiology pharmacology protein chemistry cytology immunology neurobiology neuroscience pure n applied sciences such as mass n heat transfer, kinetics, biocatalysts, bbiomechanics, bioinformatics. separation n purification processes ,bioreactor design, surface science, fluid mechanics, thermodynamics, polymer science. explain all d fields wid recpect to where dey r being applied in biotechnology!!!!
How does perfusion chromatography work?
Perfusion chromatography is a techniquearisedto overcome the problem associated with mass transfer in the separationof largemolecules such as proteins by high-performanceliquid chromatography (HPLC). Perfusion media are constituted bytwo set of pores:• throughpores(6000--8000 A°)•diffusive pores(800--1500 A°) which enable better accessof.Macromoleculesto the inner of the particle by the combination of convective and diffusive flow.Generalcharacteristics ofperfusionchromatographyIn conventional liquid chromatography (diffusion chromatography) molecules move to the outer surface of the stationary phase particles by convection which constitutes a rapid step Nevertheless, transport of molecules through a stationary phase particle occurs by molecular diffusion which constitutes a very slow process, especiallyin the case of peptides and proteins that present high molecular weights and low diffusion coefficients . As a consequence, a significant increase in bandspreading (loss in resolution) could occur.In perfusion chromatography (flow-through particle chromatography), stationary phase particlesused are designed to enable a better access of molecules to the inner of these through two classes of pores:throughpores(6000–8000 A°), which crossdamthestationary phase particle from side to side and allow the transport of molecules into the interior of the particle by convective flow anddiffusive pores(800–1500 A°), interconnecting thethroughporenetwork and enabling the transport by diffusion In this way, molecules travel by convectionthrough the column to the stationary phase particle, such as in conventional chromatography. Since there, molecules cross the stationary phase particles by means of a combination of convective and diffusive transport, thus, accelerating the transport of molecules through the particle. This singular bimodal structure significantly enhances mass transport of molecules...
