The culture which contain an organism (bacterial colony) which you are required to grow in a broth media that is a media lacking solidifying agent agar. A pure culture should not contain other bacterial or fungal cells in it except the required or cultured one
To inoculate a fungal culture in broth, a sterile loop or swab is used to transfer a small amount of the fungal culture onto the surface of the broth. The culture is then incubated at the appropriate temperature for the particular fungal strain being cultured. After incubation, the growth of the fungus in the broth can be observed and analyzed.
Cannot say without seeing the broth.
LB broth.
faculative anaerobe
Broth culture is a liquid growth medium used to grow microorganisms. It provides a nutrient-rich environment for the microorganisms to multiply. Broth culture can be easily sampled for further testing or analysis. It is commonly used in microbiology labs for cultivating and studying bacteria.
Broth culture is a type of liquid medium containing nutrients that supports the growth and multiplication of microorganisms, such as bacteria. It is commonly used in laboratories for cultivating and studying microbial species.
Transferring culture from an open broth tube to a sterile broth tube introduces microorganisms from the original culture into the sterile environment. If the transfer is done aseptically, the sterile broth will facilitate the growth of the introduced microorganisms, leading to an increase in their population. However, any contamination from the open tube could also introduce unwanted microbes, potentially affecting the purity and results of the culture. Proper aseptic techniques are crucial to minimize contamination during this process.
agar
To mixed the culture broth during fermentation
Culture broth is continually mixed to ensure uniform distribution of oxygen and nutrients, as well as to prevent sedimentation of cells or particles. This mixing promotes optimal growth conditions for the microorganisms in the broth, leading to higher productivity in fermentation processes.
Broth has been a part of various cultures for centuries, with its origins dating back to ancient times. Broth has historically been used as a nourishing and comforting food source, with different cultures developing their own variations of broths based on available ingredients and culinary traditions.
use glucose phosphate broth , inoculate the organism in that broth and incubate at 37 degree celsius for 24 hrs.