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In microbiology...
I believe it is an enzyme in some bacteria that metabolize Simmon's citrate as their sole carbon source, and not sugar. it's used for identification among bacteria. If it has this ability, it can release ammonioa and increase the pH of the medium. A color change to blue indicates it is citrate positive.
anything in science that ends in "ase" is an enzyme.
What else can I help you with?
Account for the ability of streptococci to tolerate 02 in the absence of catalase activity?
It means that they can facilitative anaerobes that can survive in both environments in aerobic and anaerobic. Citrate gets broken down by citrase producing oxaloacetic acid and acetate.
Account for the development of alkalinity in cultures capable of using citrate as their sole carbon source?
Citrate is acted on by the enzyme citrase, which produces oxaloacetic acid and acetate. These products are then enzymatically converted to pyruvic acid and carbon dioxide. During this reaction the meduim becomes alkaline (the carbon dioxide that is generated combines with sodium and water to form sodium carbonate, and alkaline product.
Why pH of Simmon's citrate agar increase if citrate is utilized?
This is due to the production of oxalacetic acid and acetate, when the enzyme citrase acts on the citrate. the oxalacetic acid and acetate is enzymatically converted to pyruvic acid and carbon dioxide. the carbon dioxide combines with sodium and water forming sodium carbonate which in turns makes the medium alkaline.
