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You can identify a gene from a DNA sequences by finding specific patterns and making predictions based on them. Biological computer programs can pinpoint patterns and help aid gene prediction.
Wiki User
∙ 9y ago
Wiki User
∙ 8y agoOne way is, they find genes by finding sites for RNA polymerase.
By checking the coding sequences, with the start and stop codons.
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How are gene of interest identified and cloned?
The expression of a gene of interest can be ensured by combining it with a gene recessive to it.
What would be the advantages of inserting into a plasmid gene with a highly visible phenotype?
Inserting a plasmid gene into the organism gives us three situation that one is the foreign cell may not pick up the plasmid the second chance is it is picked up may not expressed and in the third case it is expressed and therefore you can have the gene of interest. This is the one main advantage of studying the gene of interest by inserting a plasmid gene.
What molecules are used to extract the gene of interest and recombine the DNA?
restriction enzyme
How does DNA technology transfer bacterial genes from one cell to another?
Gene transfer between cells generally consists of the following steps: 1. Isolating the gene of interest - Here, the gene which has to be transferred has to be isolated from the genome of the source (or host) organism. 2. Splicing the gene if interest into a plasmid. Splicing is a process wherein a foreign strand of DNA (the gene of interest) is inserted into a loop of DNA called a plasmid. The plasmid DNA is cut open to form a linear fragment. The gene of interest is then attached to the plasmid DNA. The plasmid DNA is converted back into the loop form with the help of an enzyme called DNA ligase. 3. Gene amplification: Here, the plasmid containing the gene of interest is amplified. Which means, many copies of the plasmid containing DNA are created through a process called the polymerase chain reaction. 4. Transfection: This is the final step wherein the plasmid containing DNA is inserted into the recipient organism. Sometimes the foreign DNA remains within the plasmid and is able to express protein. Other times, the gene of interest can be engineered to contain a sequence called the recombination sequence which will allow it to integrate (or join) the host genome through a process called homologous recombination. By the method described above, a foreign gene is removed from one organism and inserted into another. If the gene of interest is integrated into the host of the recipient organism, copies of it are made every time the host cells divide.
How can large quantities of protein be produced from a bacterial colony containing the gene of interest?
In Gene clonning copy no of gene increse and translation of each gene produce more no of protein so one can increas production of protein
How are gene of interest identified and cloned?
The expression of a gene of interest can be ensured by combining it with a gene recessive to it.
What the role of interest rate in economic and what the good and bad effect of interest rate.?
what's effect on plabmid when gene of interest large size
Screening of cells involves?
culturing cells to find out which took the gene of interest.
What would be the advantages of inserting into a plasmid gene with a highly visible phenotype?
Inserting a plasmid gene into the organism gives us three situation that one is the foreign cell may not pick up the plasmid the second chance is it is picked up may not expressed and in the third case it is expressed and therefore you can have the gene of interest. This is the one main advantage of studying the gene of interest by inserting a plasmid gene.
What is a competition assay?
A completion assay is an experiment in which the cell growth and death of two cell lines, one with the gene of interest silenced and the other used as a control, are compared to draw conclusions about the effect of knocking down the gene of interest.
What molecules are used to extract the gene of interest and recombine the DNA?
restriction enzyme
How does DNA technology transfer bacterial genes from one cell to another?
Gene transfer between cells generally consists of the following steps: 1. Isolating the gene of interest - Here, the gene which has to be transferred has to be isolated from the genome of the source (or host) organism. 2. Splicing the gene if interest into a plasmid. Splicing is a process wherein a foreign strand of DNA (the gene of interest) is inserted into a loop of DNA called a plasmid. The plasmid DNA is cut open to form a linear fragment. The gene of interest is then attached to the plasmid DNA. The plasmid DNA is converted back into the loop form with the help of an enzyme called DNA ligase. 3. Gene amplification: Here, the plasmid containing the gene of interest is amplified. Which means, many copies of the plasmid containing DNA are created through a process called the polymerase chain reaction. 4. Transfection: This is the final step wherein the plasmid containing DNA is inserted into the recipient organism. Sometimes the foreign DNA remains within the plasmid and is able to express protein. Other times, the gene of interest can be engineered to contain a sequence called the recombination sequence which will allow it to integrate (or join) the host genome through a process called homologous recombination. By the method described above, a foreign gene is removed from one organism and inserted into another. If the gene of interest is integrated into the host of the recipient organism, copies of it are made every time the host cells divide.
What is transient expression?
Transient expression is a temporary expression of a gene or protein in a cell or organism, typically achieved using vectors or systems that do not integrate the DNA into the host genome. This allows for rapid and high-level production of the desired product without permanently modifying the host cell's genome. Transient expression is often used for research purposes or for producing recombinant proteins.
What is a good Gene Autry movie?
"The Phantom Empire" is a twelve part serial that you may find of interest .
How can large quantities of protein be produced from a bacterial colony containing the gene of interest?
In Gene clonning copy no of gene increse and translation of each gene produce more no of protein so one can increas production of protein
Why is cloning an example of cloning?
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
Why is there a need to break down the membrane during DNA extraction?
Simply to reach on DNA and extract the gene of interest.
