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What are the two broad areas of use and two examples after a host cell grown in culture to form a clone of cells containing the cloned gene of interest?
The two broad areas of use for cloned cells containing a gene of interest are medical research and biotechnology. Examples include using the cells to study gene function in disease models and to produce recombinant proteins for therapeutic purposes.
What is orf in plasmid vector and role in cloning of gene of interest?
ORF stands for Open Reading Frame, which is a sequence of nucleotides that can be translated into a protein. In a plasmid vector, an ORF can be used to clone a gene of interest by inserting the gene sequence into the ORF region, allowing the production of the corresponding protein. The ORF acts as a template for protein synthesis, enabling the expression of the cloned gene in a host organism.
What are expression vectors in recombinant DNA technology?
Expression vectors are plasmids used to produce (heterologous expression) proteins from your gene of interest in the expression host(such as E.coli, Yeast, Human cell lines). The gene of interest cloned in this vector (at the MCS) will be transformed in to the host for protein expression. check this out for more info:
Probes for cloned genes use?
Probes for cloned genes are usually short, single-stranded DNA or RNA molecules that are complementary to specific sequences in the cloned gene. These probes are labeled with a detectable marker, such as a fluorescent dye or a radioactive isotope, to allow for visualization and identification of the gene. The probes can be used in techniques like Southern blotting or in situ hybridization to detect the presence and location of the gene in a sample.
How are genes extracted from animals?
Genes can be extracted from animals using various techniques such as polymerase chain reaction (PCR) or recombinant DNA technology. Typically, a sample containing the target DNA is collected from the animal, and then specific methods are used to isolate and amplify the gene of interest. Once extracted, the gene can be analyzed, modified, or cloned for further study or applications.
What are the two broad areas of use and two examples after a host cell grown in culture to form a clone of cells containing the cloned gene of interest?
The two broad areas of use for cloned cells containing a gene of interest are medical research and biotechnology. Examples include using the cells to study gene function in disease models and to produce recombinant proteins for therapeutic purposes.
What was the name of the first cloned?
The first "cow" (it was actually a calf) in the world to be cloned was named Gene.
How can one effectively clone a gene into a plasmid?
To effectively clone a gene into a plasmid, the gene of interest and the plasmid are cut with the same restriction enzymes to create compatible ends. The gene is then inserted into the plasmid using DNA ligase to seal the ends. The plasmid is then introduced into a host cell, such as bacteria, where it can replicate and express the cloned gene.
What is orf in plasmid vector and role in cloning of gene of interest?
ORF stands for Open Reading Frame, which is a sequence of nucleotides that can be translated into a protein. In a plasmid vector, an ORF can be used to clone a gene of interest by inserting the gene sequence into the ORF region, allowing the production of the corresponding protein. The ORF acts as a template for protein synthesis, enabling the expression of the cloned gene in a host organism.
What are expression vectors in recombinant DNA technology?
Expression vectors are plasmids used to produce (heterologous expression) proteins from your gene of interest in the expression host(such as E.coli, Yeast, Human cell lines). The gene of interest cloned in this vector (at the MCS) will be transformed in to the host for protein expression. check this out for more info:
How do you predict a PCR product size from a gene cloned into an expression vector?
It all depends on where you primers are. Presumably you will have one primer that sits on the cloned gene and one that sits on the vector (that way you only get a product if the gene has cloned successfully). As long as you know where your primers land, it should be easy to work out how big the PCR product will be simply by adding the distance from the primer on the gene to the end of the gene and the distance from the primer on the vector to the end of the vector.
What is gene cloning and why do you need to clone a gene?
Gene cloning is the technique of recombinant DNA technology in which a desired gene of interest having a striking characteristic feature is cloned. The gene may be selected because it appears to influence the organism in a striking manner, or to determine the role of the gene in the organism. Genes can be clones for industrial purposes, for instance the production of vaccines and insulin, or for research purposes, to determine what the role of the gene is. Gene cloning requires a basic knowledge of the gene's sequence, or flanking sequences. Genes can be cloned using polymerase chain reaction (PCR), if the sequence is known, or by cutting genomic DNA with restriction enzymes (to create smaller chunks of DNA). Usually, once a fragment containing gene has been identified using restriction enzymes, it is sequenced and PCR is used to isolate the specific sequence within the fragment.
A single isolated gene can be cloned MOST rapidly using genetically engineered?
bacteria
How were the first cow cloned?
The information is unknown how the first cloned cow was made. The first known cloned cow was named Gene and was cloned on February 7, 1997.
Why is it easier to perform a test for huntington's disease now that the gene has been cloned?
we can map and sequence it
Antibiotic in gene cloning?
If antibiotic resistance is added to the gene being cloned, antibiotics can be used to isolate the transformed bacteria (ones with the gene being cloned) by killing off all non-transformed bacteria, that don't have the antibiotic resistance. There is a chance that the non-transformed bacteria can mutate to develop antibiotic resistance.
Does a cloned organism have one gene?
no, it has the exact same amount of genes as the original, and the genes are exactly the same
