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Gene cloning is the technique of recombinant DNA technology in which a desired gene of interest having a striking characteristic feature is cloned. The gene may be selected because it appears to influence the organism in a striking manner, or to determine the role of the gene in the organism.
Genes can be clones for industrial purposes, for instance the production of vaccines and insulin, or for research purposes, to determine what the role of the gene is.
Gene cloning requires a basic knowledge of the gene's sequence, or flanking sequences. Genes can be cloned using polymerase chain reaction (PCR), if the sequence is known, or by cutting genomic DNA with restriction enzymes (to create smaller chunks of DNA). Usually, once a fragment containing gene has been identified using restriction enzymes, it is sequenced and PCR is used to isolate the specific sequence within the fragment.
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Why is it important to isolate and clone diseased gene?
Isolating and cloning a diseased gene allows researchers to study the gene's function and effects on the body, leading to a better understanding of the disease. This information can help in the development of targeted therapies and personalized medicine for individuals affected by the disease.
How has the definition of cloning changed from the past to present?
In the past, cloning was mainly understood as the process of creating genetically identical copies of an organism. Today, the definition of cloning has expanded to include various techniques such as therapeutic cloning and gene cloning, which are used for a range of applications in biotechnology and medicine. Additionally, advancements in technology have made it possible to clone animals for agricultural and research purposes.
How does shotgun cloning differ from the clone-by-clone method in genetic engineering techniques?
Shotgun cloning involves randomly breaking up the DNA of an organism and then piecing it back together, while the clone-by-clone method involves isolating and sequencing individual DNA fragments before assembling them in a specific order. Shotgun cloning is faster but less precise, while the clone-by-clone method is slower but more accurate in genetic engineering techniques.
How many ways of cloning are there?
There are three main ways of cloning: gene cloning, reproductive cloning, and therapeutic cloning. Gene cloning involves replicating specific genes or DNA sequences, while reproductive cloning aims to create an identical copy of an organism. Therapeutic cloning is used to create stem cells for medical purposes.
Where does new DNA come from in gene cloning?
New DNA molecules can come from various sources in gene cloning, such as PCR amplification of a specific gene, synthesis of a gene using recombinant DNA technology, or isolation of a gene from a donor organism. These DNA molecules are then inserted into a vector, such as a plasmid, to create a recombinant DNA molecule for cloning.
Why is cloning an example of cloning?
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
Is cloning an organism the same as cloning a gene?
No, cloning an organism involves producing genetically identical copies of the entire organism. Cloning a gene involves making copies of a specific gene sequence. Both processes use similar techniques, such as recombinant DNA technology, but the scale and complexity differ.
Why should you use therapeutic cloning?
to clone a clincially important gene(to give protein)..eg:Insulin,glucocon,other factors
A noun from to clone?
to clone is to copy
Why is gene cloning known as a shotgun approach?
If you tray to clone something half the time it may as well be approching a shotgun, KABOOM! dead.
The cloning of many genes in hopes that at least one of the clonies of clones will contain the desired gene is referred to as the?
recombinant clone or chimeric DNA
Why is it important to isolate and clone diseased gene?
Isolating and cloning a diseased gene allows researchers to study the gene's function and effects on the body, leading to a better understanding of the disease. This information can help in the development of targeted therapies and personalized medicine for individuals affected by the disease.
What are the bad things about cloning?
It is believed that it will give the clone a shorter life span
Would a transgenic mouse be a clone?
Cloning and transgenesis are the most recent reproduction technologies. Whereas a cloned animal is genetically identical to the one from which it came, a transgenic animal is one into which a new gene has been introduced or in which an existing gene has been modified by human intervention.Thus cloning, potentially decrease genetic diversity of a population whereas transgenic animals increase the genetic diversity of a population.
What is another term for gene?
This is also known as cloning (in Biotechnology) and differs from the common perception of a clone being a replica of the original parent, although the process of cloning is directed at producing such a replicate (which may be partial or total).
What plant cloning is applied?
clone
What are the main advantages and disadvantages of cloning?
The advantage of cloning is replacing or to clone organs for a specific need although it is illegal to clone an entire human body. The disadvantage is that cloning humans would be able to create custom built people by combining the possibilities in cloning and genetic engineering. The entire body will be the same and also it will effect population too.
