answersLogoWhite

0

What is a negative stain?

Updated: 8/11/2023
User Avatar

Wiki User

15y ago

Best Answer

The negative staining techniques uses a dye solution in which the chromogen is acidic and carries a negative charge. (An acidic chromogen gives up a hydrogen ion, which leaves it with a negative charge.) The negative charge on the bacterial surface repels the negatively charged chromogen, so the the cell remains unstained against a colored background.

User Avatar

Wiki User

14y ago
This answer is:
User Avatar
More answers
User Avatar

Wiki User

15y ago

It is a staining technique that uses a dye to stain the background but not the cell. and are used to demonstrate bacterial capsule.

This answer is:
User Avatar

User Avatar

Wiki User

12y ago

A black ink such as nigrosin is usually used for negative staining.

This answer is:
User Avatar

User Avatar

Wiki User

11y ago

They stain the background, leaving the cells colorless.

This answer is:
User Avatar

User Avatar

Wiki User

14y ago

be careful in the lab

This answer is:
User Avatar

User Avatar

Wiki User

12y ago

Nigrosin is used.

This answer is:
User Avatar

Add your answer:

Earn +20 pts
Q: What is a negative stain?
Write your answer...
Submit
Still have questions?
magnify glass
imp
Related questions

What is the gram stain for cholera?

Gram Negative


Is uranyl acetate a negative stain?

Yes, uranyl acetate is used as a negative stain in electron microscopy.


Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the pH is?

8


Is HIV gram stain positive or negative?

The answer to whether HIV gram-stain positive or negative is that HIV gram-stain is negative. They retain the light red or pink color after the stain.


What kind of stain would you use to visualize Sars?

Negative stain


Why is the technique called negative staining?

Because the cell wall repels the binding of the negative stain therefore the cells do not stain. Because of this the background is stain with the dye used and the bacteria remain colorless. Basically your staining the background, that is, you are not directly staining the cells.


Why is the size more accurate in a negative stain than a simple stain?

since you do not heat fix the slide when you use a negative stain the cells do not shrink or become distorted


What does a negative result for a spore stain indicate about the organism?

A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.


What color is Gram- stain?

Gram- negative bacteria turns red to pink after the gram stain is performed.


What is shigella dysenteriae gram stain?

Negative


What Gram stain is acetobacter?

Gram negative


What is the gram stain of enterobacter cloacae?

Negative