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A phase-difference microscope (or phase-contrast microscope or phase microscope) allows better viewing of clear specimens that usually need to be stained before you can make out the various parts. Staining is a time consuming process and kills the cells being viewed. With a phase-difference microscope, living cells can be studied as they go through the cell division cycle.

HOW IT WORKS

As light goes through a transparent specimen, there may not be anything interesting to see. However, the light undergoes phase changes as it passes through the different parts of the specimen. Unfortunately, the human eye does not notice phase changes.

A phase-difference microscope converts these unnoticed phase changes into differences in brightness, which the human eye sees very well. The darker and lighter parts of the specimen make it very visible - and it is still alive!

This also works on reflected light microscopes.

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13y ago

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