to fix the color of the stain
Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).
Methylene blue
Periodic acid-Schiff's reagent (PAS) - - pink - mucins, glycogen, glycocalyx - magenta By: Wikipedia
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
to fix the color of the stain
Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).
8
It's simple, because it's a stain.
Mordant is the reagent used in the gram stain method. By using this type of reagent bacteria are more easily seen. It is important to a doctor to know what kind of bacteria is present so that he knows what to prescribe to a patient to get rid of the bacteria.
A simple stain like iodine can make cell parts show up that would otherwise be nearly invisible since they are colorless.A simple stain like iodine will reveal a cell's morphology.
schiller's iodine solution
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
simple answer is NO
You can see clearer images in the simple stain technique rather than the wet mount technique...
alek mading
Reagent bottles are containers made of glass or plastic, and are closed by special caps or stoppers, and are intended to contain chemicals in liquid or powder form for laboratories and stored in cabinets or on shelves.